Efficacy of Environmental DNA to Detect and Quantify Brook Trout Populations in Headwater Streams of the Adirondack Mountains, New York
By B. Baldigo, Lee Ann Sporn, S. George, Jacob A. Ball. 2017
114 citations recorded by the source corpus.
AbstractEnvironmental DNA (eDNA) analysis is rapidly evolving as a tool for monitoring the distributions of aquatic species. Detection of species’ populations in streams may be challenging because the persistence time for intact DNA fragments is unknown and because eDNA is diluted and dispersed by dynamic hydrological processes. During 2015, the DNA of Brook Trout Salvelinus fontinalis was analyzed from water samples collected at 40 streams across the Adirondack region of upstate New York, where Brook Trout populations were recently quantified. Study objectives were to evaluate different sampling methods and the ability of eDNA to accurately predict the presence and abundance of resident Brook Trout populations. Results from three-pass electrofishing surveys indicated that Brook Trout were absent from 10 sites and were present in low ( 300 fish/0.1 ha) densities at 9, 11, and 10 sites, respectively. The eDNA results correctly predicted the prese...
Subfields: Ecology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/bedc51014ad60a627d4677d9e0911dcddbdcd003
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Citations in the corpus
- Environmental DNA (eDNA) as a tool for assessing fish biomass: A review of approaches and future considerations for resource surveys (2021, methodology): “…the substrate used (water/sediment) and method of eDNA capture, extraction, storage, eDNA primer design and validation, and PCR amplification (Baldigo et al., 2017; Eichmiller et al., 2016; Furlan et al., 2016; Hinlo et al., 2017b; Jane et al., 2015; Knudsen et al., 2019; Kumar et al., 2020;…”