Interaction of colloidal gold nanoparticles with human blood: effects on particle size and analysis of plasma protein binding profiles

By M. Dobrovolskaia, A. Patri, Jiwen Zheng, J. Clogston, N. Ayub, Parag Aggarwal, B. Neun, Jennifer B Hall, S. McNeil. Nanomedicine: Nanotechnology, Biology and Medicine, 2008

665 citations recorded by the source corpus.

Nanoparticle size and plasma binding profile contribute to a particle’s longevity in the bloodstream, which can have important consequences for therapeutic efficacy. In this study an approximate doubling in nanoparticle hydrodynamic size was observed upon in vitro incubation of 30- and 50-nm colloidal gold in human plasma. Plasma proteins that bind the surface of citrate-stabilized gold colloids have been identified. Effects of protein binding on the nanoparticle hydrodynamic size, elements of coagulation, and the complement system have been investigated. The difference in size measurements obtained from dynamic light scattering, electron microscopy, and scanning probe microscopy are also discussed.

Subfields: Biomaterials, Electronic, Optical and Magnetic Materials, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f26247381db21e0821d415aa7f99aa742fdcd234

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Citations in the corpus

Toxicity and cellular uptake of gold nanoparticles: what we have learned so far? (2010, positive): “Encouragingly, citrate-capped gold nanoparticles (spheres of diameter 30 and 50 nm) have been shown to be ‘‘blood compatible’’ and did not induce any detectable platelet aggregation, change in plasma coagulation time, or immune response in at least one study (Dobrovolskaia et al. 2009).”
Effects of cell culture media on the dynamic formation of protein-nanoparticle complexes and influence on the cellular response. (2010, result): “only partially interact with the NP surface (Figure 5).(18,21,36) 39 Such an effect was found to be similar in”
Gold Nanoparticles in Diagnostics and Therapeutics for Human Cancer (2018, background): “3700 proteins, and the gold nanoparticles that come in contact with the plasma form a “protein corona complex” containing fewer than 100 proteins [24].”
Protein corona fingerprinting predicts the cellular interaction of gold and silver nanoparticles. (2014, background): “Upon adsorption, the anionic serum proteins impart a net negative charge to the nanoparticles.(34,44) 48”
Dynamic light scattering for gold nanorod size characterization and study of nanorod–protein interactions (2012, methodology): “From a literature survey, we found that the most commonly used method is fluorescence spectroscopy [16, 21, 22].”