Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels

By U. Phutela, V. Dhuna, S.K. Sandhu, B. S. Chadha. 2005

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A thermophilic fungal strain producing both pectinase and polygalacturonase was isolated after primary screening of 120 different isolates. The fungus was identified as Aspergillus fumigatus Fres. MTCC 4163. Using solid-state cultivation, the optimum levels of variables for pectinase and polygalacturonase (PG) production were determined. Maximal levels of enzyme activities were achieved upon growing the culture in a medium containing wheat bran, sucrose, yeast extract and (NH4)2SO4 after 2-3 days of incubation at a temperature of 50oC. Highest enzyme activities of 1116 Ug-1 for pectinase and 1270 Ug-1 for polygalacturonase were obtained at pH 4.0 and 5.0, respectively.

Subfields: Biomedical Engineering, Biotechnology, Plant Science

View Paper: https://www.semanticscholar.org/paper/f2332e7f2740b1d47e0574731c06995fe03b82fc

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