Production and Characterization of Pectinase Enzyme from Penicillium chrysogenum

By A. Banu, M. Devi, G. R. Gnanaprabhal, B. Pradeep, M. Palaniswamy. 2010

90 citations recorded by the source corpus.

Ten moulds isolated from municipal waste soil sample were screened for pectinolytic enzyme production when grown on pectin containing (YPSS) solid media. Penicillium chrysogenum was selected based on clearance zones and pectinase enzyme production was carried out in submerged fermentation. Enzyme production by Penicillium chrysogenum was higher at pH 6.5 and a temperature of 35°C using sucrose and ammonium per sulphate as carbon source and nitrogen source, respectively. The maximal activity of P. chrysogenum pectinase was at 50°C, pH 6.5 and was thermostable up to 40°C. MgCl 2 and CaCl 2 ions had little effect on pectinase activity. K m and V max values were 1.0 mg/mL and 85 U/mg protein, respectively and an apparent molecular weight of 31 kDa on SDS-PAGE.

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Citations in the corpus

• Beneficial fungal communities from different habitats and their roles in plant growth promotion and soil health (2020, background): “Fungi like Thermomucor indicae seudaticae (Martin et al. 2010), Penicillium chrysogenum (Banu et al. 2010), A. japonicus and P. glandicola (Bezerra et al. 2012), A. niger (Ahmed et al. 2016; El Enshasy et al. 2018; Geetha et al. 2012; Ortiz et al. 2017; Sandri et al. 2013; Wang et al. 2019) and A.…”