Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles

By T. Baumgart, A. Hammond, Prabuddha Sengupta, Samuel T. Hess, D. Holowka, B. Baird, W. Webb. Proceedings of the National Academy of Sciences of the United States of America, 2007

798 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/f1b2f5590efc19229844c7a909ce6f9bcc19660a

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Citations in the corpus

Revitalizing membrane rafts: new tools and insights (2010, background): “So if one were to leave a plasma membrane in a resting state (no lipid synthesis or metabolism, no exocytosis or endocytosis, no interactions with peripheral proteins and no actin cytoskeleton), would membrane components phase-separate into large domains with time? This question has yet to be answ”
Membrane organization and lipid rafts. (2011, background): “Baumgart et al. (2007) showed that cells treated with paraformaldehyde and dithiothreitol produced membrane blebs that could be isolated as giant plasma membrane vesicles.”
Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis (2011, background): “The proteins also had different lipid partitioning characteristics in giant plasma-membrane vesicles (GPMVs”
Co-operation of TLR4 and raft proteins in LPS-induced pro-inflammatory signaling (2014, background): “In these plasma membrane fragments, separation of Lo and Ld phases accompanied by accumulation of selected membrane proteins in the ordered phase can be observed [47, 49–51].”