Creation of low-copy integrated transgenic lines in Caenorhabditis elegans.

By V. Praitis, Elizabeth Casey, D. Collar, J. Austin. Genetics, 2001

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Single-copy insertion of transgenes in Caenorhabditis elegans (2008, methodology): “An alternative method for generating stable transgenes is biolistic transformation using DNA-coated gold particle”
TrackMate: An open and extensible platform for single-particle tracking. (2017, methodology): “If this process is imaged using a fluorescent strain labeled for nuclei [24], harsh imaging conditions will perturb the normal development and hinder normal progression.”
Nictation, a dispersal behavior of the nematode Caenorhabditis elegans, is regulated by IL2 neurons (2011, methodology): “, and integration lines were made by the bombardment technique as describe”
Spindle Dynamics and the Role of γ-Tubulin in Early Caenorhabditis elegans Embryos (2001, methodology): “The bombardment method of Praitis et al. (2001) was used to generate a strain containing pie-1::GFP::b-tubulin (from pJH4.”
Transformation and microinjection (2006, methodology): “For transformation, the relatively new technique of gene bombardment is fast becoming a popular alternative because transgenes are often integrated into the genome at low copy number, which offers a number of advantages as described below (Praitis et al., 2001; Berezikov et al., 2004).”