Carrier-bound Immobilized Enzymes: Principles, Application and Design

By Linqiu Cao. 2005

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Immobilization strategies to develop enzymatic biosensors. (2012, background): “Many books and comprehensive reviews have been written on enzyme immobilization thus reporting thousands of protocols (Cao, 2005a, 2005b; Guisan, 2006; Hermanson et al., 1992; Hermanson, 2008; Mulchandani and Rogers, 1998; Mateo et al., 2007; Minteer, 2011).”
Recent advance in the support and technology used in enzyme immobilization (2009, background): “In the industrial biotechnology, the skill to stabilize and re-use an enzyme catalyst through immobilization has proven one to be of the key steps in rendering an enzymatic process that is economically viable (Cao and Schmid, 2005; Zhou, 2009).”
An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes (2015, neutral): “[9] Depending on the pH of the solution and the isoelectric point, the surface of the enzyme may bear charges [56] and its charge distribution can be readily calculated and displayed using current available modelling systems.”