High-Throughput Sequencing Technologies

By Jason A. Reuter, Damek V. Spacek, Michael Snyder. Molecules and Cells, 2015

1,194 citations recorded by the source corpus.

Summary The human genome sequence has profoundly altered our understanding of biology, human diversity and disease. The path from the first draft sequence to our nascent era of personal genomes and genomic medicine has been made possible only because of the extraordinary advancements in DNA sequencing technologies over the past ten years. Here, we discuss commonly used high-throughput sequencing platforms, the growing array of sequencing assays developed around them as well as the challenges facing current sequencing platforms and their clinical application.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/eea80bb8e08722ff4cb852d2d4ba0ce25ccd14e0

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In Molecular Biology

Citations in the corpus

Reactome pathway analysis: a high-performance in-memory approach (2017, background): “Since the size of the data samples is constantly increasing [2, 3], Reactome offers a set of pathway analysis tools which aim to deal with this scenario and yet provide reliable and accurate results with interactive (seconds) response time for genome-wide datasets.”
Oral squamous cell carcinomas: state of the field and emerging directions (2023, background): “Already, oral cancer treatment has undergone a substantial transformation, leading to improved patient outcomes.(480) A multidisciplinary team will be required to manage these tumors, consisting not only of surgeons and oncologists, but also of specialists evaluating the nutritional, mental, social,”
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, methodology): “In comparison, the Ion Torrent machine is a more expensive instrument (perhaps comparable to the cost of an Illumina NextSeq machine), capable of producing up to 10 Gb of data per run, but the sequences are shorter, at just 200 bp (Reuter et al. 2015).”
JASPAR 2024: 20th anniversary of the open-access database of transcription factor binding profiles (2023, background): “…effort is the rapid expansion witnessed following the introduction of high-throughput sequencing assays such as ChIP-seq ( 21 ), DAP-seq ( 22 ), PBM ( 23 ), SMiLE-seq ( 24 ), and HT-SELEX ( 25 ), which accelerated the generation of datasets suitable for modelling TF-DNA interactions ( 26 ,27 ).”
UALCAN: A Portal for Facilitating Tumor Subgroup Gene Expression and Survival Analyses (2017, background): “Recent advances in high throughput technologies such as next-generation sequencing (NGS) and microarrays have enabled basic, translational and clinical cancer researchers to investigate molecular changes in DNA, RNA, and proteins at high throughput scale [1 – 3].”
Generations of Sequencing Technologies: From First to Next Generation (2017, background): “The process continues with the elimination of the terminator with the fluorescent label and the starting of a new cycle with a new incorporation [21,39].”
Advances in the Application of Genetics in Marine Turtle Biology and Conservation (2017, background): “These technologies are rapidly advancing, and detailed evaluation of these methodologies is available elsewhere (Morey et al., 2013; Reuter et al., 2015).”
Microbial Biodiversity and Bioremediation Assessment Through Omics Approaches (2020, background): “Rhee et al. (2004) established an extensive 50-mer-based oligonucleotide microarray to identify genes associated with biodegradation and biotransformation in microbial communities.”