Complete Chemical Synthesis , Assembly , and Cloning of a Mycoplasma genitalium Genome

By K. Maeda, Koji Kawabata, P. Mazzali, S. Valenti, K. Nomoto. 2008

322 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/ed98633e0e82312f6e791bee10693a67d14fa71e

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Citations in the corpus

A Whole-Cell Computational Model Predicts Phenotype from Genotype (2012, unknown): “…the recent de novo synthesis of the M. genitalium chromosome and successful genome transplantation of Mycoplasma genomes to produce a synthetic cell (Gibson et al., 2008, 2010; Lartigue et al., 2007, 2009), raise the exciting possibility of using whole-cell models to enable computer-aided rational…”
A Synthetic Genetic Edge Detection Program (2009, background): “The construction of very large fragments of DNA (Cello et al., 2002; Chan et al., 2005; Endy, 2008; Gibson et al., 2008a; Gibson et al., 2008b; Smith et al., 2003; Tumpey et al., 2005) is no longer a limitation in the engineering of biological systems.”
DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways (2008, background): “genitalium genome from 101 fragments (29) indicates that it is possible to assemble a DNA molecule as large as a genome from short DNA fragments using DNA assembler.”
Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides (2009, background): “Previously, we showed that six overlapping dsDNA fragments could be assembled by yeast into an entire Mycoplasma genitalium genome (17).”
The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes (2012, background): “While all of these issues may be ultimately resolved by the synthesis of entire genomes à la carte (21), the immediate future still needs better molecular tools for the following: (i) exploration, analysis and de-construction of the functional space of existing bacteria; (ii) reconstruction of the s”
Engineering Modular Viral Scaffolds for Targeted Bacterial Population Editing (2015, methodology): “Inspired by gap-repair cloning in yeast (Ma et al., 1987) and the pioneering work of Gibson and co-workers (Gibson, 2012; Gibson et al., 2008; Gibson et al., 2009), we captured phage genomes into Saccharomyces cerevisiae, thus enabling facile genetic manipulation of modified genomes that can be…”
A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. (2015, unknown): “…E. A., Baden-Tillson, H., Zaveri, J., Stockwell, T. B., Brownley, A., Thomas, D. W., Algire, M. A., Merryman, C., Young, L., Noskov, V. N., Glass, J. I., Venter, J. C., Hutchison, C. A., and Smith, H. O. (2008) Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.”
Enzymatic Assembly of Overlapping DNA Fragments (2011, methodology): “…method for assembling overlapping DNA molecules makes use of the 30-exonuclease activity of T4 DNA polymerase (T4 pol) to produce ssDNA overhangs, and a combination of Taq DNA polymerase (Taq pol) and Taq DNA ligase (Taq lig) to repair the annealed joints (Gibson et al., 2008, 2009; Fig.”