Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells

By N. Kulak, G. Pichler, Igor Paron, Nagarjuna Nagaraj, Matthias Mann. Nature Methods, 2014

1,618 citations recorded by the source corpus.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/ed76c3063caac945397d46020664d5e6286744f0

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Citations in the corpus

Protein Synthesis Initiation in Eukaryotic Cells. (2018, methodology): “Shown in Table 1 is a subset of the data obtained by Kulak et al. (2014), using a label-free mass spectrometry protocol (numbers rounded off ) for exponentially growing yeast and HeLa cell cultures, thereby enabling comparison across species from a single study.”
Online Parallel Accumulation–Serial Fragmentation (PASEF) with a Novel Trapped Ion Mobility Mass Spectrometer (2018, positive): “One-device cell lysis, reduction, and alkylation was performed in sodium deoxycholate (SDC) buffer with chloroacetamide (PreOmics GmbH, Martinsried, Germany) according to our previously published protocol (32).”
A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation. (2015, positive): “HeLa cells were lysed in guanidinium chloride lysis buffer and digested sequentially with LysC and trypsin (Kulak et al., 2014).”
Multidimensional proteomics for cell biology (2015, background): “Such speed and sensitivity has, for example, facilitated the analysis of the whole proteome of an organism in less than 24 hours 14,15 .”
Plasma Proteome Profiling to Assess Human Health and Disease. (2016, methodology): “Sample Preparation: Protein Digestion and iST Purification Sample preparation was performed as described previously (Kulak et al., 2014) with optimization for blood plasma as follows: 24 ml of SDC reduction and alkylation buffer (Kulak et al., 2014) were added to 1 ml of blood plasma.”
SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation (2017, methodology): “These cleanup procedures can result in substantial losses of protein, and colleagues have developed advanced methods, such as SP3 [23] and iST [24], that minimize protein losses and allow for”