Differential effects of resistance and endurance exercise in the fed state on signalling molecule phosphorylation and protein synthesis in human muscle
By S. B. Wilkinson, Stuart M Phillips, P. Atherton, Rekha C. Patel, K. Yarasheski, M. Tarnopolsky, M. Rennie. Journal of Physiology, 2008
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Resistance (RE) and endurance (EE) exercise stimulate mixed skeletal muscle protein synthesis. The phenotypes induced by RE (myofibrillar protein accretion) and EE (mitochondrial expansion) training must result from differential stimulation of myofibrillar and mitochondrial protein synthesis. We measured the synthetic rates of myofibrillar and mitochondrial proteins and the activation of signalling proteins (Akt–mTOR–p70S6K) at rest and after an acute bout of RE or EE in the untrained state and after 10 weeks of RE or EE training in young healthy men. While untrained, RE stimulated both myofibrillar and mitochondrial protein synthesis, 67% and 69% (P < 0.02), respectively. After training, only myofibrillar protein synthesis increased with RE (36%, P= 0.05). EE stimulated mitochondrial protein synthesis in both the untrained, 154%, and trained, 105% (both P < 0.05), but not myofibrillar protein synthesis. Acute RE and EE increased the phosphorylation of proteins in the Akt–mTOR–p70S6K pathway with comparatively minor differences between two exercise stimuli. Phosphorylation of Akt–mTOR–p70S6K proteins was increased after 10 weeks of RE training but not by EE training. Chronic RE or EE training modifies the protein synthetic response of functional protein fractions, with a shift toward exercise phenotype‐specific responses, without an obvious explanatory change in the phosphorylation of regulatory signalling pathway proteins.
Subfields: Cell Biology, Molecular Biology, Orthopedics and Sports Medicine
View Paper: https://www.semanticscholar.org/paper/eca867c5001962e45ce8194ddd30c560fd972fb2
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Citations in the corpus
- Exercise metabolism and the molecular regulation of skeletal muscle adaptation. (2013, background): “An acute bout of resistance exercise results in an increased rate ofmixed,mitochondrial, andmyofibrillar protein synthesis during recovery (Biolo et al., 1995; Phillips et al., 1997; Wilkinson et al., 2008) and a proportionately smaller increase in the protein degradation rate (Biolo et al.”
- Age‐related differences in the dose–response relationship of muscle protein synthesis to resistance exercise in young and old men (2009, methodology): “Myofibrillar protein was isolated as previously described (Wilkinson et al. 2008).”