Morpholino oligos: making sense of antisense?

By J. Heasman. Developmental Biology, 2002

597 citations recorded by the source corpus.

Since morpholino oligos were first introduced as a means to inhibit gene function in embryos, in the Spring of 2000, they have been tested in a range of model organisms, including sea urchin, ascidian, zebrafish, frog, chick, and mouse. This review surveys the results of these studies and examines the successes and limitations of the approach for targeting maternal and zygotic gene function. The evidence so far suggests that, with careful controls, morpholinos provide a relatively simple and rapid method to study gene function.

Subfields: Cell Biology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ec578c80f7d1943065fe7a46031783866b5744b8

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Citations in the corpus

Controlling morpholino experiments: don't stop making antisense (2008, background): “, 1995), the use of conventional antisense RNA, antisense oligonucleotides, or RNAi has not met with success in understanding the roles of zygotically expressed genes (Heasman, 2002).”
Zebrafish as a model vertebrate for investigating chemical toxicity. (2005, background): “…although an even distribution of MO through the zebrafish has been observed after injection (Nasevicius and Ekker, 2000), it is important to stress that phenotypes induced by MOs can vary considerably in severity, and injection of the oligo can cause nonspecific adverse effects (Heasman, 2002).”
Loss-of-function approaches in comparative physiology: is there a future for knockdown experiments in the era of genome editing? (2019, methodology): “…to be capable of phenocopying null mutant phenotypes in frogs (Heasman et al., 2000) and zebrafish (Nasevicius and Ekker, 2000), and rapidly became an important tool for assessing gene function in biological research (Ekker, 2000; Corey and Abrams, 2001; Heasman, 2002; Bill et al., 2009).”
In vivo drug discovery in the zebrafish (2005, background): “Gene function can be rapidly and robustly studied in zebrafish using antisense MORPHOLINO OLIGONUCLEOTIDES”
Antisense technologies. Improvement through novel chemical modifications. (2003, background): “Recently, the success and limitations of their usage have been reviewed comprehensively, with particular focus on developmental biology [56] as most published work on morpholino compounds has been carried out using zebrafish embryos.”