Direct observation of base-pair stepping by RNA polymerase

By E. Abbondanzieri, W.J. Greenleaf, Joshua W. Shaevitz, R. Landick, S. Block. Nature, 2005

858 citations recorded by the source corpus.

Subfields: Atomic and Molecular Physics, and Optics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/eb9150f1b56356ea7ea9d9538477623813a626da

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In Atomic and Molecular Physics, and Optics

In Molecular Biology

Citations in the corpus

Stochastic mechano-chemical kinetics of molecular motors: A multidisciplinary enterprise from a physicist’s perspective (2012, background): “High quality single-molecule data [1508] as well as high-resolution structures obtained from X-ray crystallography [1509] are consistent with this Brownian ratchet mechanism and inconsistent with the power stroke mechanism.”
Super resolution fluorescence microscopy (2009, background): “High-precision localization of bright light sources, such as fluorescent particles, is often used in biophysical experiments (31,32) and has reached a precision as high as ~1 Å (33).”
Shaping the future of manipulation (2011, methodology): “This is the foundation of the trapped particle being a revolutionary tool for single-molecule biophysic”
Kinesin motor mechanics: binding, stepping, tracking, gating, and limping. (2007, background): “rectly to a measurement of the step size (Abbondanzieri et al., 2005), but in others, it corresponds instead to the distance to a transition state, which is always less than the step size.”
Colloquium: Physical approaches to DNA sequencing and detection (2007, background): “…of the effective charge of DNA in solution, optical traps have provided the necessary control and precision force measurements for the study of DNA interacting with RNA polymerase at the single-base level (Abbondanzieri et al., 2005) and even single-molecule sequencing (Greenleaf and Block, 2006).”
Multiple optical trapping and binding: new routes to self-assembly (2010, background): “Very recently, angstrom level displacement has been achieved with an ultra-stable apparatus to explore the key process of transcription where RNA polymerase (RNAP) moves processively along a DNA template, creating a complementary RNA [41].”
A review of imaging techniques for systems biology (2008, methodology): “In [39,40], an optical trapping system was used to make angstrom resolution measurements of pair based stepping of RNA polymerase, and thus has established an important resolution benchmark in molecular biology.”
Nanometric optical tweezers based on nanostructured substrates (2008, background): “For these reasons, nanometric trapping of bio-objects requires a rather complicated optical set-u”
Review on Cell Mechanics: Experimental and Modeling Approaches (2013, neutral): “…have also been used to investigate the forces generated by molecular motors [40,188–195], those created by individual RNA polymerase molecules during transcription [196] [197] [198] [199] [200], and those developed during protein unfolding/folding [201] [202] [203] or binding/unbinding [204] [205].”
Optical tweezers for single cells (2008, background): “4 Å (Abbondanzieri et al. 2005; Greenleaf et al. 2005). Rohrbach (2005) has measured a controlled 25 fN force on a 533 nm latex sphere.”
Nonequilibrium fluctuations in small systems: From physics to biology (2007, background): “Current optical tweezer techniques have even resolved the motion of the enzyme at the level of a single base pair [23, 24].”
SINGLE-PARTICLE TRACKING (2009, background): “4 the Bohr radius of the hydrogen atom) [80].”