Multiplexed particle-based flow cytometric assays.

By D. Vignali. JIM - Journal of Immunological Methods, 2000

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The Time of Prenatal Immune Challenge Determines the Specificity of Inflammation-Mediated Brain and Behavioral Pathology (2006, methodology): “Cytokine protein levels in the maternal serum and fetal brain tissue were measured using a multiplexed particle-based flow cytometric cytokine assay (Vignali, 2000).”
In‐field molecular diagnosis of plant pathogens: recent trends and future perspectives (2018, background): “A Luminex xMAP analyser excites the internal dyes of the microspheres with a red laser and the reporter fluorochrome, captured by the antigen, with a green laser, allowing the specific detection of multiple analytes at the same time (Vignali, 2000; Charlermroj et al., 2013).”
Simultaneous Detection of 15 Human Cytokines in a Single Sample of Stimulated Peripheral Blood Mononuclear Cells (2003, methodology): “The Bio-Plex system employing the Luminex multianalyte profiling technology (Lab-MAP) allows individual and multiplex analysis of up to 100 different analytes in a single microtiter well (20).”
Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis (2007, methodology): “Among those, the multiplex analysis system, which uses a combination of fluorescently dyed microspheres associated with a two-laser flow cytometry based system [13], permits the simultaneous analysis of up to 100 different biomolecules (proteins, peptides, or nucleic acids) in a single microplate we”