Signal transduction in maize and Arabidopsis mesophyll protoplasts.

By Jen Sheen. Plant Physiology, 2001

697 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/eaa429fdf965839f969850913783149c3322e75c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Z. Ye
2. R. Zhong
3. Jianli Zhou
4. Chanhui Lee
5. E. Richardson
6. V. S. Lin
7. B. Trewyn
8. Frederick M. Ausubel

In Plant Science

1. Z. Ye
2. R. Zhong
3. Jianli Zhou
4. Chanhui Lee
5. E. Richardson
6. M. B. Bitonti
7. Maria Greco
8. A. Chiappetta

Citations in the corpus

• Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis (2007, background): “More general information about plant protoplasts can be found in a revie”
• The MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis (2007, methodology): “duced into Arabidopsis leaf protoplasts by polyethylene glycol‐mediated transfection according to Sheen (2001) .”
• Sugar Sensing and Signaling in Plants Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010455. (2002, background): “Further characterization of the auxin and cytokinin signaling components (Guilfoyle et al., 1998; Gray and Estelle, 2000; Hwang and Sheen, 2001; Mok and Mok, 2001) should reveal the molecular mechanisms that underlie their interactions with the sugar signal transduction pathway.”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, positive): “Protein localization AtZIP1 and AtZIP2 were cloned into the pSAT1 enhanced green fluorescent protein (eGFP) expression vector in-frame with the C-terminus of eGFP using the KpnI site, and transiently expressed in freshly isolated protoplasts from 3- to 4-week-old Arabidopsis seedlings according to S”
• A simple and effective method for protein subcellular localization using Agrobacterium-mediated transformation of onion epidermal cells (2007, background): “How- ever, the suspension-cultured cells and protoplasts as well as the stable transformation are time-consuming, expensive and need high proficiency of special techniques and great patience (Scott et al. 1999; Sheen 2001).”
• MYB58 and MYB63 Are Transcriptional Activators of the Lignin Biosynthetic Pathway during Secondary Cell Wall Formation in Arabidopsis[C][W] (2009, methodology): “The reporter and effector constructs were cotransfected into Arabidopsis leaf protoplasts (Sheen, 2001).”
• A Battery of Transcription Factors Involved in the Regulation of Secondary Cell Wall Biosynthesis in Arabidopsis (2008, methodology): “The SND1-HER expression construct was introduced into Arabidopsis leaf protoplasts alone or together with the MYB46P:GUS construct (Sheen, 2001).”
• The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species (2009, background): “Routine transient assays include biolistic bombardment [4], protoplast transfection [5], and Agrobacterium-mediated transient assays [6], each with advantages and disadvantages depending on the research goals.”
• MAP kinase signalling cascade in Arabidopsis innate immunity (2002, methodology): “Early defence gene transcription To dissect the early signal transduction pathways in plant innate immune responses, we first established a leaf cell assay based on flg22 inducible transcription of early response genes in Arabidopsis mesophyll protoplast”
• Mesoporous silica nanoparticles deliver DNA and chemicals into plants. (2007, methodology): “We first investigated the use of MSN on protoplasts (plant cells with the cell wall removed), which are often used as model systems in physiological and cellular process studie”
• An efficient cucumber (Cucumis sativus L.) protoplast isolation and transient expression system (2013, background): “Therein, PEG-mediated protoplast transient expression has high transformation efficiency, so it is widely applied in plant molecular biology studies (Sheen, 2001; Walter et al., 2004).”
• A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes (2011, background): “Arabidopsis, maize [3] and tobacco protoplasts [4], tobacco leaf epidermal cells [5], tobacco BY-2 cells [6] and onion epidermal cells [7] are commonly used for transient assays in gene expression, protein subcellular localization, protein-protein interaction and protein activity studies.”
• Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis (2004, methodology): “Transient expression analyses were performed with Arabidopsis mesophyll protoplast as described by Sheen (2001).”