Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis

By S. Yoo, Young-Hee Cho, Jen Sheen. Nature Protocols, 2007

4,583 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/b2ef5573520aaad3f977bf80f8290437e18627c1

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Jian-Feng Li
2. Federico Manuel Giorgi
3. Laurentius A. C. J. Voesenek
4. Francesco Licausi
5. D. A. Weits
6. P. Perata
7. B. Giuntoli
8. Monika Kosmacz

In Plant Science

1. Jian-Feng Li
2. Laurentius A. C. J. Voesenek
3. G. Striker

Citations in the corpus

• An efficient cucumber (Cucumis sativus L.) protoplast isolation and transient expression system (2013, methodology): “8) (Yoo et al., 2007) and mixed gently, then filtered through nylon membrane (200 mesh).”
• A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes (2011, methodology): “PEG-mediated transfections were carried out as described [8].”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “The resultant plasmid has the”
• Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization (2011, methodology): “Protoplasts were prepared according to a previously described metho”
• The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species (2009, background): “Protoplast transfection also works for diverse plant species and could be used to investigate cell-autonomous regulation and responses in a quantitative and high-throughput way [7].”