Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation

By K. Kiick, Eliana Saxon, D. Tirrell, Carolyn R. Bertozzi. Proceedings of the National Academy of Sciences of the United States of America, 2001

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Gelatinase-mediated migration and invasion of cancer cells. (2005, background): “Alternatively, novel functional groups such as fluorescent probes (Cornish et al., 1994), amino acids with side chains containing chemically modifiable groups (Kiick et al., 2002) or photoaffinity labels for cross-linking (Chin et al., 2002) can be added.”
Site-specific antibody drug conjugates for cancer therapy (2013, methodology): “In addition to the example of pAcPhe described above, other unnatural amino acids are also under investigation for use in site-specific conjugation using similar processes involving matching tRNA/aminoacyl-tRNA synthetase pairs.(57,58) In vitro transcription and translation methods were recently dev”
Chemistry in living systems (2005, methodology): “Similarly, a methionine auxotroph was used for production of proteins that contained homopropargylglycine or azidohomoalanine at sites that encode for methionin”