A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein

By J. Nakai, Masamichi Ohkura, K. Imoto. Nature Biotechnology, 2001

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Motor control in a Drosophila taste circuit (2009, methodology): “The top image is initial G-CaMP fluorescence, bottom is change in fluorescence (DF/F) to 100 mM sucrose.”
Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain. (2003, methodology): “…upon imaging The imaging system we have developed couples the of sensory and projection neurons suggests the faithful high sensitivity of G-CaMP (Nakai et al., 2001) with twotransmission of sensory input to higher brain centers. photon microscopy (Denk et al., 1990) to allow the detec- These…”
Imaging Neural Activity Using Thy1-GCaMP Transgenic mice (2012, methodology): “Imaging experiments were performed 36–48 hr after the transfection as described previously (Nakai et al., 2001).”