Chemical derivatization and mass spectral libraries in metabolic profiling by GC/MS and LC/MS/MS.

By J. Halket, D. Waterman, A. Przyborowska, Raj K. P. Patel, P. Fraser, P. Bramley. Journal of Experimental Botany, 2005

628 citations recorded by the source corpus.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/e7a8d072bb1ebe134965ce047cec252602dfdef6

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Citations in the corpus

Metabolomics technology and bioinformatics (2006, background): “(iii) metabolic fingerprinting (Table 1) [26, 27].”
Global metabolic profiling procedures for urine using UPLC–MS (2010, background): “Many of the analytes present in urine are polar, ionic and relatively involatile and, as a result, require complex and lengthy sample preparation and derivatization to obtain samples that are both suitable for injection onto the GC column and that contain volatile analytes (a useful review of deriva”
Metabolomics for plant stress response. (2008, background): “Themajor advantage ofGC-MS formetabolomics is the availability of both commercially and publicly available EI spectral libraries (Halket et al. 2005).”
Comparative Metabolome Analysis of the Salt Response in Breeding Cultivars of Rice (2007, background): “…hand profiling is biased towards those metabolic components from a study that are identified at present, for example those that have been made publicly available in dedicated electronic libraries (Halket et al. 1999, Wagner et al. 2003, Halket et al. 2005, Kopka et al. 2005, Schauer et al. 2005).”
Decision tree supported substructure prediction of metabolites from GC-MS profiles (2010, methodology): “For this reason, hit list based mass spectra similarity matching has evolved as a highly successful tool for the routine assessment of mass spectra (Halket et al. 2005), and large commercial mass spectral libraries, such as NIST08 (http://chemdata.nist.gov/mass-spc/Srch_v1.7/index.html) have been…”
Mass spectrometry-based metabolomics. (2007, background): “It has also to be noted that silylation can cause conversion reactions, for example, it has been described that arginine is converted into ornithine by reaction with BSTFA or MSTFA (Halket et al., 2005).”
Organization of GC/MS and LC/MS metabolomics data into chemical libraries (2010, background): “The first step is to begin a binning process where ions from samples in the same study set are binned by mass and retention index (RI) [23].”
The use of metabolomics to dissect plant responses to abiotic stresses (2012, methodology): “The crucial advantage of this technology is that it has long been used for metabolite profiling, and thus there are stable protocols for machine setup and maintenance, and chromatogram evaluation and interpretation [9–11].”