MtDNA barcode identification of fish larvae in the southern Great Barrier Reef, Australia

By G. Pegg, B. Sinclair, Leica Briskey, W. Aspden. 2006

155 citations recorded by the source corpus.

Planktonic larvae were captured above a shallow coral reef study site on the Great Barrier Reef (GBR) around spring-summer new moon periods (October-February) using light trap or net capture devices. Larvae were identified to the genus or species level by comparison with a phylogenetic tree of tropical marine fish species using mtDNA HVR1 sequence data. Further analysis showed that within-species HVR1 sequence variation was typically 1-3%, whereas between-species variation for the same genus ranged up to 50%, supporting the suitability of HVR1 for species identification. Given the current worldwide interest in DNA barcoding and species identification using an alternative mtDNA gene marker (cox1), we also explored the efficacy of different primer sets for amplification of cox1 in reef fish, and its suitability for species identification. Of those tested, the Fish-F1 and -R1 primer set recently reported by Ward et al. (2005) gave the best results.

Subfields: Ecology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e531e150ba1bc4d27eeadb3dd102c430acdf1e31

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Citations in the corpus

Using DNA barcodes to connect adults and early life stages of marine fishes from the Yucatan Peninsula, Mexico: potential in fisheries management (2010, background): “MtDNA barcode identification of fish larvae in the southern Great Barrier Reef, Australia.”
DNA barcodes of fish of the Scotia Sea, Antarctica indicate priority groups for taxonomic and systematics focus (2008, background): “…support confirming the utility of DNA barcoding (Hebert et al. 2003) is amassing at a rapid rate for a wide range of organisms (Waugh 2007), and recent studies have shown that barcoding can identify a large variety of fish species (Steinke et al. 2005, Ward et al. 2005, Pegg et al. 2006).”
Molecular identification methods of fish species: reassessment and possible applications (2009, background): “In the past 5 years, several studies have evaluated the potential of DNA barcoding for identifying fish species, among which Australia’fishes (Ward et al. 2005; Pegg et al. 2006) (for a more complete review about DNA barcoding progresses see Waugh 2007).”
Females do count: Documenting Chironomidae (Diptera) species diversity using DNA barcoding (2010, background): “Several studies have demonstrated the usefulness of DNA barcodes to associate life stages in practice (e.g. Caterino and Tishechkin 2006; Pegg et al. 2006; Zhou et al. 2009); Carew et al. (2005) and Ekrem et al. (2007) have found that partial COI gene sequences can be used to link different life…”
The campaign to DNA barcode all fishes, FISH-BOL. (2009, methodology): “COI barcoding has also been used to identify fish larvae taken from the Great Barrier Reef (Pegg et al., 2006) and the Caribbean (Victor, 2007) and fish eggs from temperate Australian waters (F. Neira, pers. comm.).”
DNA barcoding as a tool for coral reef conservation (2007, background): “It is possible to obtain suYcient DNA from larvae as small as »1 mm in length for ampliWcation of COI-5 (Baldwin et al. 1996; Hare et al. 2000; Schander and Willassen 2005; Pegg et al. 2006; Richardson et al. 2006; Webb et al. 2006).”
DNA barcoding is a useful tool for the identification of marine fishes from Japan (2011, background): “The application of DNA barcoding to identify species at all life-history stages is beneficial and helpful for better understanding the ecological mechanisms (Katoh, 2001; Pegg et al., 2006; Rocha-Olivares and Chávez-González, 2008; Victor et al., 2009).”