Spatiotemporal Gene Expression Targeting with the TARGET and Gene-Switch Systems in Drosophila

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Motor control in a Drosophila taste circuit (2009, methodology): “1 (Baines et al., 2001); tub-Gal80ts (McGuire et al., 2004); UAS-ChR2::YFP (a gift from Steven Stowers); UAS-mCD8::GFP (Lee and Luo, 1999); UAS-mCD8:: dsRed (Ye et al., 2007); UAS-dsRed; tub > Gal80 > (see below); hs-FLP, MKRS (Bloomington stock center); UAS > CD2,y+ > -mCD8::GFP (Wong et al.,…”
Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut (2009, methodology): “To express cell death effectors, we used esgGal4 and the temperature-sensitive Gal4 repressor, tubGal80ts (McGuire et al., 2004) (together referred to as esgts), to allow temporal activation of UAS-linked target genes in ISCs and EBs (Jiang and Edgar, 2009; Micchelli and Perrimon, 2006).”
Keap1/Nrf2 signaling regulates oxidative stress tolerance and lifespan in Drosophila. (2008, methodology): “The genetically identical progeny of such crosses were separated by gender after one day of mating, and each gender was further split into two groups that were maintained for 48 hours on either RU486-containing (320 μM) or control food (corresponding amount of ethanol) (McGuire et al., 2004).”
Converging circuits mediate temperature and shock aversive olfactory conditioning in Drosophila. (2014, neutral): “1 [48, 49];”