Primer3 on the WWW for general users and for biologist programmers.

By S. Rozen, H. Skaletsky. Methods in molecular biology, 2000

16,780 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/e26b2804e4d12e0391893c496d46fdbd832dd1a2

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In Molecular Biology

Citations in the corpus

Parallel metatranscriptome analyses of host and symbiont gene expression in the gut of the termite Reticulitermes flavipes (2009, positive): “Rozen S, Skaletsky HJ: Primer3 on the WWW for general users and for biologist programmers.”
Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction (2012, methodology): “Notably, the widely used Primer3 program [7] designs primers based on a variety of parameters.”
Development and characterization of polymorphic microsatellite loci in endangered fern Adiantum reniforme var. sinense (2006, methodology): “0 program (Rozen and Skaletsky 2000) and used for preliminary assessment.”
MethPrimer: designing primers for methylation PCRs (2002, methodology): “In this paper, we introduce a new program called MethPrimer that was based on the well-known primer design program Primer3 (Rozen and Skaletsky, 2000) and was developed specifically for bisulfite-conversion-based PCRs.”
Water-extractable humic substances alter root development and epidermal cell pattern in Arabidopsis (2007, methodology): “Primers were designed by the Primer3 program (Rozen and Skaletsky 2000).”
DNA-based identification of Alaska skates (Amblyraja, Bathyraja and Raja: Rajidae) using cytochrome c oxidase subunit I (coI) variation (2006, methodology): “New primers were designed using Primer3 software (Rozen & Skaletsky, 2000) and the complete mtDNA sequence from Raja radiata Donovan, 1808 (Genbank accession number NC000893).”
IDT SciTools: a suite for analysis and design of nucleic acid oligomers (2008, methodology): “PrimerQuest is based on the Primer3 code (37).”
Construction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences (2008, positive): “Tri- and tetranucleotide were favoured over dinucleotide repeats, and the length of the SSR motif was more than 12 bp. Primer pairs were designed for 130 SSRs using Primer3 ( Rozen and Skaletsky 2000 ).”
Database resources of the National Center for Biotechnology Information (2014, methodology): “Primer-BLAST is a tool for designing and analyzing polymerase chain reaction (PCR) primers based on the existing program Primer3 (56) that designs PCR primers given a template DNA sequence.”
Transport Distance of Invertebrate Environmental DNA in a Natural River (2014, neutral): “0 [24] from pre-existing sequence data available from the NCBI nucleotide database (Table 1, Dataset S1 and S2) [25].”