Arteriolar niches maintain haematopoietic stem cell quiescence
By Y. Kunisaki, I. Bruns, C. Scheiermann, Jalal Ahmed, Sandra Pinho, Dachuan Zhang, T. Mizoguchi, Qiaozhi Wei, D. Lucas, Keisuke Ito and 3 more. Nature, 2013
1,161 citations recorded by the source corpus.
Cell cycle quiescence is a critical feature contributing to haematopoietic stem cell (HSC) maintenance. Although various candidate stromal cells have been identified as potential HSC niches, the spatial localization of quiescent HSCs in the bone marrow remains unclear. Here, using a novel approach that combines whole-mount confocal immunofluorescence imaging techniques and computational modelling to analyse significant three-dimensional associations in the mouse bone marrow among vascular structures, stromal cells and HSCs, we show that quiescent HSCs associate specifically with small arterioles that are preferentially found in endosteal bone marrow. These arterioles are ensheathed exclusively by rare NG2 (also known as CSPG4)+ pericytes, distinct from sinusoid-associated leptin receptor (LEPR)+ cells. Pharmacological or genetic activation of the HSC cell cycle alters the distribution of HSCs from NG2+ periarteriolar niches to LEPR+ perisinusoidal niches. Conditional depletion of NG2+ cells induces HSC cycling and reduces functional long-term repopulating HSCs in the bone marrow. These results thus indicate that arteriolar niches are indispensable for maintaining HSC quiescence.
Subfields: Cell Biology, Hematology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/dfc557e2b24db1d6850141f42e316d471197e712
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Cell Biology
In Hematology
In Molecular Biology
Citations in the corpus
- Leptin Receptor-expressing mesenchymal stromal cells represent the main source of bone formed by adult bone marrow (2014, positive): “Furthermore, Nestin-GFP cells are heterogeneous, including both Nestin-GFP cells that localize mainly around arterioles and Nestin-GFP cells that localize mainly around sinusoids, raising questions about the distribution of HSC niche factors and MSCs among these cell populations (Kunisaki et al., 20”