A resource for large-scale RNA-interference-based screens in mammals

By Patrick J. Paddison, Jose M. Silva, D. Conklin, M. Schlabach, Mamie Z. Li, Shola Aruleba, Vivekanand S. Balija, A. O'Shaughnessy, L. Gnoj, Kimberly N. Scobie and 7 more. Nature, 2004

723 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/de9048ff13a282c6a91ba5e1e415f63cc92b81ef

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Citations in the corpus

RNA silencing: Methods and protocols (2006, methodology): “As in other eukaryotes, interference involves digestion of the interfering double-stranded RNA into short fragments (2), a polysome-associated complex (3), and Argonaute protein (4,5).”
BAC TransgeneOmics (2008, background): “The temperature is shifted to 37 1C and expression of the proteins required for recombination is induced with L-arabinose (2).”
Analysis of cell-based RNAi screens (2006, background): “elegans to elucidate whole organism phenotypes and for cell-based assays in fly, mouse, and human cells [8-17].”
Large‐scale image‐based screening and profiling of cellular phenotypes (2017, background): “Genome-wide siRNA or CRISPR-Cas9 libraries for human genes have been developed (48,49).”
The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo (2011, background): “We have further generated genomewide shRNA libraries in these vectors that cover large numbers of mammalian transcripts for systematic study of gene function (5, 6).”
Integrative genomic approaches identify IKBKE as a breast cancer oncogene. (2007, background): “Indeed, several large RNAi libraries now exist and have been used to examine the function of genes in a high-throughput manner (Berns et al., 2004; Paddison et al., 2004), including those necessary for cancer cell viability (Moffat et al.”
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation (2016, background): “Highly multiplexed pooled genetic screening methodologies have emerged as powerful and broadly accessible tools for systematically profiling gene function at the scale of mammalian genomes (Paddison et al., 2004).”