Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches

By Mo Chen, J. Manley. Nature reviews. Molecular cell biology, 2009

1,287 citations recorded by the source corpus.

Alternative splicing of mRNA precursors provides an important means of genetic control and is a crucial step in the expression of most genes. Alternative splicing markedly affects human development, and its misregulation underlies many human diseases. Although the mechanisms of alternative splicing have been studied extensively, until the past few years we had not begun to realize fully the diversity and complexity of alternative splicing regulation by an intricate protein–RNA network. Great progress has been made by studying individual transcripts and through genome-wide approaches, which together provide a better picture of the mechanistic regulation of alternative pre-mRNA splicing.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/dcbb801e132bd1ce2ae3cce74e4ebaf057325614

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Citations in the corpus

Alternative Splicing Regulatory Networks: Functions, Mechanisms, and Evolution. (2019, background): “When binding at these positions, SR proteins generally oppose the repressive effects of hnRNPs (Chen and Manley, 2009); for example, SRRM4 opposes the repressive effects of PTBP1 on the inclusion of microexons (GonatopoulosPournatzis et al.”
Dido3-dependent SFPQ recruitment maintains efficiency in mammalian alternative splicing (2019, background): “Most accessory SF have a function in positioning spliceosome subunits on the primary transcript, but are recycled immediately after initiation of the splicing reactions (9,46).”
iCLIP reveals the function of hnRNP particles in splicing at individual nucleotide resolution (2010, background): “Splice-site selection is primarily mediated by RNA-binding proteins that bind regulatory elements within nascent transcript”
A highly conserved program of neuronal microexons is misregulated in autistic brains (2014, background): “The assembly of spliceosomes at 50 and 30 splice sites is typically regulated by RNA-binding proteins (RBPs) that recognize proximal cis-elements, referred to as exonic/intronic splicing enhancers and silencers (Chen and Manley, 2009).”
Mechanisms and Regulation of Alternative Pre-mRNA Splicing (2015, background): “Regarding alternative splicing, both single-gene and genome-wide methods have led to important insights into how alternative splicing patterns are set up and maintained in particular cell or tissue types (9, 14, 15).”
MATS: a Bayesian framework for flexible detection of differential alternative splicing from RNA-Seq data (2012, background): “Most splicing regulators may control the splicing of up to several hundred exons via sequence-specific protein– RNA interactions (35).”
Complexity of the Alternative Splicing Landscape in Plants[C][W][OPEN] (2013, background): “SREs SREs are short (5 to 10 nucleotides) conserved sequences that often occur in clusters (Chen and Manley, 2009; McManus and Graveley, 2011).”