NINJA connects the co-repressor TOPLESS to jasmonate signalling

By L. Pauwels, Gemma Fernández Barbero, J. Geerinck, Sofie Tilleman, W. Grunewald, A. C. Pérez, J. Chico, R. V. Bossche, J. Sewell, Eduardo Gil and 7 more. Nature, 2010

953 citations recorded by the source corpus.

Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes. JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. On perception of JA-Ile, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated. The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the Arabidopsis JAZ proteins recruit the Groucho/Tup1-type co-repressor TOPLESS (TPL) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). NINJA acts as a transcriptional repressor whose activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress-related and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants.

Subfields: Insect Science, Plant Science

View Paper: https://www.semanticscholar.org/paper/dc230cf7da823d2c4322f305d97735c70b5a2be1

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Insect Science

1. C. Wasternack
2. B. Hause
3. C. Pieterse
4. S. He
5. Jonathan D. G. Jones
6. G. Howe
7. Bethany Huot
8. Beronda L. Montgomery

In Plant Science

1. Kazuko Yamaguchi-Shinozaki
2. Kazuo Shinozaki
3. C. Wasternack
4. M. Fujita
5. Y. Fujita
6. C. Pieterse
7. Motoaki Seki
8. R. Finkelstein

Citations in the corpus

• Hormonal modulation of plant immunity. (2012, background): “The adaptor protein NOVEL INTERACTOR OF JAZ (NINJA) interacts with the ZIM domain of most JAZ proteins (Pauwels et al. 2010).”
• Alterations of Endogenous Hormonal Levels in Plants under Drought and Salinity (2016, background): “In spite of JA and MeJA were previously thought to be key regulators of JAs responses induced by stresses, it has been demonstrated that the isoleucine conjugate of JA (JA-Ile) acts in the signal transduction pathway of JA regulating stress-responses [44].”
• ABA-mediated transcriptional regulation in response to osmotic stress in plants (2011, background): “More recently, it was proposed that the interaction of the NINJA-related AFP proteins with ABI5 and co-repressor TPL-related proteins (TPRs) might be implicated in the regulation of ABI5-dependent gene expression (Pauwels et al. 2010).”
• Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis (2011, background): “It was recently reported that JAZs interact with an adaptor protein NINJA that recruits Groucho/Tup1-type corepressor TOPLESS and its related proteins to suppress MYC2-dependent transcription (28).”
• The Arabidopsis bHLH Transcription Factors MYC3 and MYC4 Are Targets of JAZ Repressors and Act Additively with MYC2 in the Activation of Jasmonate Responses[C][W] (2011, background): “They are also important regulators of growth and developmental programs such as gamete development, the cell cycle, root growth, tendril coiling, and senescence in many plant species (Pauwels et al., 2008; Zhang and Turner, 2008; Reinbothe et al., 2009; Yoshida et al., 2009).”
• Role of phytohormones in insect-specific plant reactions (2012, background): “The co-repressor TOPLESS (TPL) interacts with EAR-motif (ethylene-responsive element binding factor-associated amphiphilic repression) proteins to repress transcription of genes involved in several hormone pathways [71].”
• Abscisic Acid Synthesis and Response (2013, background): “, 2010 ), and a clade of proteins of unknown function (AFPs) that have been proposed to destabilize ABI5 and/or interact with the co-repressor TOPLESS (TPL: AT1G15750) to inhibit expression of ABI5-regulated genes (Lopez-Molina et al., 2003; Garcia et al., 2008; Pauwels et al., 2010).”
• Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany. (2013, methodology): “The domain structure of MYC2, Jasmonate ZIM domain proteins (JAZ) and Novel Interactor of JAZ (NINJA) (A) and a hypothetical scheme of interaction between MYC2, JAZ, NINJA and TOPLESS (TPL) (B).”
• Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade (2012, background): “This transcriptional repression requires novel interactor of JAZ (NINJA) and TOPLESS corepressor proteins (11).”
• The JAZ Proteins: A Crucial Interface in the Jasmonate Signaling Cascade (2011, background): “, Pauwels, L., De Jaeger, G., and Goossens, A. (2010).”