Autoinhibition and activation mechanisms of the Wiskott–Aldrich syndrome protein

By Annette S. Kim, L. Kakalis, N. Abdul-Manan, Grace A. Liu, M. Rosen. Nature, 2000

779 citations recorded by the source corpus.

Subfields: Cardiology and Cardiovascular Medicine, Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/db9aa1b8bde06ae96c48a87c1df38f307051174d

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Citations in the corpus

Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck (2002, background): “Phosphatidylinositol(4,5)bisphosphate (PIP2) can further activate N-WASP in cooperation with NCK or Cdc42 by binding to a basic region of N-WASP.”
Calmodulin in action: diversity in target recognition and activation mechanisms. (2002, background): “CaM has been also shown to play a prominent induced structures according to the context of different role in modulating the hallmark feedback inhibition or binding partners (Kim et al., 2000).”
Analysis of molecular recognition features (MoRFs). (2006, background): “Interestingly, the Arp2/3 binding activity of the VCA MoRF is autoinhibited by the GDB MoRF (Figure 3(e)).(72) This auto-inhibitory interaction is interrupted by binding of the GDB MoRF to activated Cdc42 (Figure 3(d)),(73) which releases the VCA MoRF to interact with Arp2/3.”
SH3 domains: complexity in moderation. (2001, background): “Ladbury and Arold have argued that there is insufficient inherent specificity in most SH3-mediated interactions to account for specific biological outputs without one having to invoke additional mechanisms (Ladbury and Arold, 2000).”
Protein Metamorphosis: The Two-State Behavior of Mad2 (2008, background): “There are also examples of certain protein domains adopting different folds when bound to different targets (Kim et al., 2000).”
Evaluation of disorder predictions in CASP5 (2003, background): “For example, the protein may interact with multiple ligands, and adopt correspondingly different structures to complement each, 3 or the functional structure of the protein may require the presence of a partner before a single conformation is of sufﬁciently low free energy to dominate the…”
Prediction of Protein Binding Regions in Disordered Proteins (2009, neutral): “. Its mutations can be linked to the Wiskott-Aldrich Syndrome (WAS), a disease characterized by actin cytoskeleton defects leading to deficiencies in blood clotting and immune response. The protein is composed of various functional domains.”
The role of the Rho GTPases in neuronal development. (2005, background): “This induces a conformational change that releases the WASP VCA domain from auto-inhibition, allowing it to activate the Arp2/3 complex to nucleate the formation of new actin filaments in vitro (Machesky et al. 1999; Rohatgi et al. 1999, 2000; Kim et al. 2000).”
Integrative Annotation of Variants from 1092 Humans: Application to Cancer Genomics (2013, background): “However, WASP uses only the PBD domain for interacting with CDC42 and regulating its auto-inhibition (73).”
Three-dimensional reconstruction of protein networks provides insight into human genetic disease (2012, background): “A n A ly s i s One well-studied example of pleiotropy is the Wiskott-Aldrich syndrome protein ( WASP , also known as WAS ) 32 , which contains a WH1 and a PBD domain ( Fig.”
Regulation of actin assembly associated with protrusion and adhesion in cell migration. (2008, background): “Cdc42 and PIP2 disrupt the intramolecular interaction to unmask the VCA domain (100, 168, 183).”