Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III

By P. Gönczy, C. Echeverri, K. Oegema, A. Coulson, Steven J. M. Jones, Richard R. Copley, J. Duperon, Jeff Oegema, M. Brehm, Etienne Cassin and 11 more. Nature, 2000

896 citations recorded by the source corpus.

Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in Caenorhabditis elegans. We inhibited the expression of ∼96% of the ∼2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an in vivo time-lapse differential interference contrast microscopy assay, we identified 133 genes (∼6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in C. elegans embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that ∼47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.

Subfields: Aging, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/da5ace75f7f435fb065677be1378b465ff5ebc4d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Aging

In Molecular Biology

Citations in the corpus

Toward automatic phenotyping of developing embryos from videos (2005, neutral): “I NTRODUCTION”
Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. (2002, neutral): “Unfortunately, RNAi genes and transgenic reporter genes can show partial does not inhibit all genes [2, 3 ]. We show that loss of resistance to RNAi in the nervous system [5, 6]. For a function of a putative RNA-directed RNA polymerase”
Genome-Wide RNAi of C. elegans Using the Hypersensitive rrf-3 Strain Reveals Novel Gene Functions (2003, methodology): “Other global RNAi screens have been recently performed in C. elegans using this RNAi library or other techniques (Gönczy et al. 2000; Maeda et al. 2001; Dillin et al. 2002; Piano et al. 2002; Ashrafi et al. 2003; Lee et al. 2003; Pothof et al. 2003).”