Broadly Reactive and Highly Sensitive Assay for Norwalk-Like Viruses Based on Real-Time Quantitative Reverse Transcription-PCR

By T. Kageyama, Shigeyuki Kojima, M. Shinohara, K. Uchida, S. Fukushi, F. Hoshino, N. Takeda, K. Katayama. Journal of Clinical Microbiology, 2003

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Diagnosing COVID-19: The Disease and Tools for Detection (2020, methodology): “33, 34 The design process generally involves two main steps: (1) sequence alignment and primer design, and (2) assay optimization and testing.”
Norovirus classification and proposed strain nomenclature. (2006, positive): “Consequently, reverse transcription-polymerase chain reaction (RT-PCR) and genomic sequencing have become the major means for characterizing the viruses and to understand the relatedness of different strains (Ando et al., 2000; Jiang et al., 1990, 1993; Katayama et al., 2002).”
Advances in Laboratory Methods for Detection and Typing of Norovirus (2014, background): “Because only one small region of the norovirus genome is sufficiently conserved for the development of genogroup-specific oligonucleotide primers and probes (25), most of the reported norovirus RT-qPCR assays target this ORF1-ORF2 junction region (26, 27).”