Absolute quantification by droplet digital PCR versus analog real-time PCR

By Christopher M. Hindson, J. Chevillet, H. Briggs, Emily N. Gallichotte, I. K. Ruf, B. Hindson, R. Vessella, Muneesh Tewari. Nature Methods, 2013

1,378 citations recorded by the source corpus.

Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased 37–86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis, this translated to superior diagnostic performance for identifying individuals with cancer.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d888204bcde877082651aed8c2d208f445b4238f

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In Biomedical Engineering

1. Phenix-Lan Quan
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1. Sean C. Taylor

Citations in the corpus

• Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients (2020, result): “This result is consistent with previous reports [15,16].”
• Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data (2017, background): “Droplet Digital PCR (ddPCR) is a recent technology that has become commercially available since 2011 9, 10 .”
• dPCR: A Technology Review (2018, methodology): “dPCR exhibited higher precision with a decreased coefficient of variation for the quantification of HIV DNA [135] or serum miRNAs [136] using droplet dPCR.”
• Quantitative and stoichiometric analysis of the microRNA content of exosomes (2014, methodology): “We recently described (38) a droplet digital PCR (ddPCR) -based approach for absolute quantification of miRNAs from biofluid samples.”