Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system
By Sueli C Marques, Amit Zeisel, Simone Codeluppi, David van Bruggen, Ana Mendanha Falcão, Lin Xiao, Huiliang Li, Martin Häring, Hannah Hochgerner, R. Romanov and 13 more. Science, 2016
1,037 citations recorded by the source corpus.
One size does not fit all Oligodendrocytes are best known for their ability to myelinate brain neurons, thus increasing the speed of signal transmission. Marques et al. surveyed oligodendrocytes of developing mice and found unexpected heterogeneity. Transcriptional analysis identified 12 populations, ranging from precursors to mature oligodendrocytes. Transcriptional profiles diverged as the oligodendrocytes matured, building distinct populations. One population was responsive to motor learning, and another, with a different transcriptome, traveled along blood vessels. Science, this issue p. 1326 Brain oligodendrocytes express transcriptional heterogeneity between brain regions and age of development. Oligodendrocytes have been considered as a functionally homogeneous population in the central nervous system (CNS). We performed single-cell RNA sequencing on 5072 cells of the oligodendrocyte lineage from 10 regions of the mouse juvenile and adult CNS. Thirteen distinct populations were identified, 12 of which represent a continuum from Pdgfra+ oligodendrocyte precursor cells (OPCs) to distinct mature oligodendrocytes. Initial stages of differentiation were similar across the juvenile CNS, whereas subsets of mature oligodendrocytes were enriched in specific regions in the adult brain. Newly formed oligodendrocytes were detected in the adult CNS and were responsive to complex motor learning. A second Pdgfra+ population, distinct from OPCs, was found along vessels. Our study reveals the dynamics of oligodendrocyte differentiation and maturation, uncoupling them at a transcriptional level and highlighting oligodendrocyte heterogeneity in the CNS.
Subfields: Cancer Research, Developmental Neuroscience, Physiology
View Paper: https://www.semanticscholar.org/paper/d73c3acd26af16ae8cb3ed3f06ea57a7f8592e22
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Citations in the corpus
- Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain (2018, background): “The first stage of analysis robustly grouped cells into 8–11 broad classes (Data S2), including neurons, astrocytes, microglia/macrophages, oligodendrocytes, polydendrocytes (oligodendrocyte progenitor cells), and components of the vasculature—endothelial cells, fibroblast-like cells, andmural cells”
- A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types (2017, methodology): “To compare oligodendrocyte clusters to maturational stages, we generated a heatmap showing single-cell expression of oligodendrocyte stage-specific marker genes identified by reference 9 .”
- Integrative single-cell analysis of transcriptional and epigenetic states in the human adult brain (2017, neutral): “Further, although snDrop-seq permits more wide-ranging tissue profiling compared to our previously published method 4 , we were unable to distinguish subpopulations of cortical astrocytes and oligodendrocytes found in mouse studies 56, 57 .”
- White matter changes in Alzheimer’s disease: a focus on myelin and oligodendrocytes (2018, neutral): “These OPCs are located along vessels and they show similarities with pericyte lineage cells [51].”
- SCENIC: Single-cell regulatory network inference and clustering (2017, background): “Note that this cancer trajectory represents a different “trajectory” compared to normal oligodendrocyte differentiation (Figure S14 for the SCENIC analysis of 5069 oligodendrocytes 58 ).”
- Cell diversity and network dynamics in photosensitive human brain organoids (2017, background): “Cluster c9 (17,103 cells) contained progenitors of neuroepithelial origin, including cells with an oligodendrocyte-precursor-like identity 20,26 .”
- Molecular Architecture of the Mouse Nervous System (2018, background): “They also expressed many markers of tanycytes of the third ventricle, including Nes, Vim, Rax and Gpr50 (Miranda-Angulo et al., 2014), but their location in the dorsal midbrain suggests that they instead represented a tanycyte of the circumventricular organs (Kettenmann and Ransom, 2013).”