Cell diversity and network dynamics in photosensitive human brain organoids
By Giorgia Quadrato, T. Nguyen, Evan Z. Macosko, J. Sherwood, S. M. Yang, D. Berger, Natalie Maria, J. Scholvin, Melissa Goldman, J. Kinney and 5 more. Nature, 2017
1,138 citations recorded by the source corpus.
In vitro models of the developing brain such as three-dimensional brain organoids offer an unprecedented opportunity to study aspects of human brain development and disease. However, the cells generated within organoids and the extent to which they recapitulate the regional complexity, cellular diversity and circuit functionality of the brain remain undefined. Here we analyse gene expression in over 80,000 individual cells isolated from 31 human brain organoids. We find that organoids can generate a broad diversity of cells, which are related to endogenous classes, including cells from the cerebral cortex and the retina. Organoids could be developed over extended periods (more than 9 months), allowing for the establishment of relatively mature features, including the formation of dendritic spines and spontaneously active neuronal networks. Finally, neuronal activity within organoids could be controlled using light stimulation of photosensitive cells, which may offer a way to probe the functionality of human neuronal circuits using physiological sensory stimuli.
Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience
View Paper: https://www.semanticscholar.org/paper/a39d38cd95664f4e45449d14d419a655117d271c
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In Cellular and Molecular Neuroscience
In Cognitive Neuroscience
Citations in the corpus
- Complex oscillatory waves emerging from cortical organoids model early human brain network development (2019, background): “Despite recent advances in the understanding of their cellular diversity, there is no evidence that these organoids develop complex and functional neural network activity that resembles early human brain formation (Birey et al., 2017; Giandomenico et al., 2019; Quadrato et al., 2017).”
- Identifying gene expression programs of cell-type identity and cellular activity with single-cell RNA-Seq (2018, methodology): “cNMF deconvolutes hypoxia and cell-cycle activity GEPs from identity GEPs in brain organoid data Having demonstrated its performance and utility on simulated data, we then used cNMF to re-analyze a published scRNA-Seq dataset of 52,600 single cells isolated from human brain organoids (Quadrato et al”
- Is It Time to Start Transitioning From 2D to 3D Cell Culture? (2020, background): “All of this is made possible by 3D cultures’ capability to model a cell in vivo while being cultured in vitro (Ravi et al., 2015).”