Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells

By V. T. Chu, Timm Weber, B. Wefers, W. Wurst, S. Sander, K. Rajewsky, R. Kühn. Nature Biotechnology, 2015

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Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering. (2016, neutral): “While making programmed cuts has become largely trivial, biasing DNA repair toward homology-directed repair rather than non-homologous end joining remains a challenge (Chu et al., 2015; Maruyama et al., 2015).”
Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018, background): “Although inhibiting the NHEJ pathway with gene silencing or chemical inhibitors can partially increase the efficiency of HDR pathway, the gene repair efficacy still needs to be further improved for disease therapy [82, 85].”
Delivering CRISPR: a review of the challenges and approaches (2018, background): “, 2015), gene silencing (Chu et al., 2015), cell cycle synchronization (Lin et al.”
Pathways to de‐extinction: how close can we get to resurrection of an extinct species? (2017, background): “Genome editing approaches are improving both in the efficiency with which they target and replace the correct region of the genome (Chu et al. 2015; Maruyama et al. 2015) and in their capacity to make multiple changes simultaneously (Yang et al. 2015).”