High Levels of Id1 Expression Define B1 Type Adult Neural Stem Cells

By Hyung-song Nam, R. Benezra. 2009

273 citations recorded by the source corpus.

Defining the molecular identity of stem cells may be critical for formulating a rational strategy for the therapeutic intervention of stem cell dysfunction. We find that high expression of Id1, a dominant-negative helix-loop-helix transcriptional regulator, identifies a rare population GFAP+ astrocytes with stem cell attributes among the subventricular astrocytes in the adult brain. The rare, long-lived, and relatively quiescent Id1high astrocytes with morphology characteristic of B1 type astrocytes self-renew and generate migratory neuroblasts that differentiate into olfactory bulb interneurons. Cultured Id1high neural stem cells can self-renew asymmetrically and generate a stem and a differentiated cell expressing progressively lower levels of Id1, revealing an Id1 gradient in unperturbed cells of subventricular neurogenic lineages. Moreover, Id genes are necessary to confer self-renewal capacity, a characteristic of stem cell identity. We suggest that high expression of a single transcriptional regulator, Id1, molecularly defines the long-sought-after B1 type adult neural stem cells.

Subfields: Developmental Neuroscience, Genetics, Molecular Biology

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Citations in the corpus

Adult Neurogenesis in the Mammalian Brain: Significant Answers and Significant Questions (2011, background): “Shh signaling is also activated in radial glia-like cells (Ahn and Joyner, 2005) and required for their establishment and maintenance in the adult SVZ and SGZ (Balordi and Fishell, 2007; Han et al., 2008).”
Prox1 Is Required for Granule Cell Maturation and Intermediate Progenitor Maintenance During Brain Neurogenesis (2010, background): “Prox1 Is Required for the Maintenance of Intermediate Progenitors in the SGZ During Adult Neurogenesis In the SGZ, Prox1 expression is initially detected in Tbr2 (Figure 6E) [35] and Dcx (Figure 6F) Type-IIb intermediate progenitors [36] and is absent from adult NSCs (Figure 6A,B,C) [37,38] and Ascl”
MEF promotes stemness in the pathogenesis of gliomas (2012, unknown): “In recent years, many studies have shown that genes involved in normal stem cell biology also play a relevant role in tumorigenesis (Nam and Benezra , 2009) (Venere et al., 2011).”
Inductive angiocrine signals from sinusoidal endothelium are required for liver regeneration (2010, unknown): “Using Id1 venusYFP reporter mice in which the venusYFP expression is driven by the Id1 promoter 24 , we found exclusive Id1 upregulation in LSECs 48 hours after PH (Fig.”