Cross-linked enzyme aggregates with enhanced activity: application to lipases

By P. López-Serrano, L. Cao, F. van Rantwijk, R. Sheldon. Biotechnology Letters, 2002

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Improvement of enzyme activity, stability and selectivity via immobilization techniques (2007, background): “he presence of detergents may permit to get improved lipase ctivity, very likely by fixing the open form of lipases durng crosslinking [105].”
Enzyme stabilization by nano/microsized hybrid materials (2013, background): “In contrast, adding of salts, water-miscible organic solvents, or nonionic polymers to enzymes results in precipitation as physical aggregates without denaturation [35].”
Immobilized Enzymes in Biosensor Applications (2019, methodology): “As an example, the precipitation of lipases from Thermomyces lanuginosus and Rhizomucor miehei with (NH4)2SO4 in the presence of sodium dodecyl sulfate (SDS), followed by cross-linking with glutaraldehyde, resulted in CLEAs with three-fold and two-fold increases in the hydrolytic activity compared w”
Advances in enzyme immobilisation (2009, unknown): “2007b) CLEA (López-Serrano et al. 2002; Sheldon 2007b) and Spherezymes (Brady et al.”