Improvement of enzyme activity, stability and selectivity via immobilization techniques

By C. Mateo, J. Palomo, G. Fernandez-Lorente, J. Guisán, R. Fernández-Lafuente. 2007

3,228 citations recorded by the source corpus.

Subfields: Electrical and Electronic Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f74f3b8eb5a0c3cc6dd4ae16360ade6bdd5ddef6

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Electrical and Electronic Engineering

In Molecular Biology

Citations in the corpus

Phytases and Phytase-Labile Organic Phosphorus in Manures and Soils (2013, background): “In other cases, enzyme activity may be even enhanced by adsorption (Cao, 2005; Mateo et al., 2007; Rosas et al., 2008).”
Strategies for the one-step immobilization-purification of enzymes as industrial biocatalysts. (2015, background): “The rigidification of certain areas of the protein’s surface and its controlled distortion resulting from the immobilization process have been shown to tune (in some instances significantly improving) enzyme activity, selectivity or specificity (Mateo et al., 2007c; Rodrigues et al., 2013).”
Polymer biodegradation: mechanisms and estimation techniques. (2008, background): “Moreover, they are also protected against autocatalytic denaturation (in particular proteases) (Mateo et al., 2007).”
Lipases from Mammals and Fishes (2009, methodology): “Various modifications have been applied to lipases in order to improve their solubility, activity, and stability in organic solvents: attaching soluble modifiers like aldehydes, polyethylene glycols, and imidoesters; adsorption onto support particles; gel entrapment; immobilization; and protein engi”
A general overview of support materials for enzyme immobilization: Characteristics, properties, practical utility (2018, background): “Moreover, after binding the enzyme molecules, the catalysts change from a homogeneous to a heterogeneous form, which facilitates simple separation of the biocatalytic system from the reaction mixture and results in products of higher purity [5,6].”
Enzyme immobilization by adsorption: a review (2014, background): “However, the enormous catalytic potential offered by the enzymes for innumerable transformations, has stimulated intense studies aimed at the improvement of their properties (Mateo et al. 2007; Brady and Jordon 2009; FernandezLafuente 2009; Garcia-Galan et al. 2011; Cowan and Fernandez-Lafuente 2011”