Influence of Hermetia illucens meal dietary inclusion on the histological traits, gut mucin composition and the oxidative stress biomarkers in rainbow trout (Oncorhynchus mykiss)

By A. Elia, M. Capucchio, Barbara Caldaroni, Gabriele Magara, A. Dörr, I. Biasato, E. Biasibetti, M. Righetti, P. Pastorino, M. Prearo and 3 more. Aquaculture, 2018

127 citations recorded by the source corpus.

Abstract Insect protein meals are considered as a promising alternative feedstuff in aquafeeds, but until now the effects induced by its dietary inclusion on histological features, gut mucin composition and oxidative stress in fish are still not assessed. In the present study, we describe these effects after inclusion of a partially defatted Hermetia illucens (HI) larvae meal in Oncorhynchus mykiss diets. Three isoproteic, isolipidic and isoenergetics diets were formulated containing HI larvae meal in partial substitution of fish meal: 0% (HI0, control diet), 25% (HI25) and 50% (HI50), corresponding to dietary inclusion levels of 0, 20% and 40% of HI. Fish were fed for 78 days with the experimental diets and at the end of the growing trial, histological and histochemical evaluations were performed on spleen, liver and gut samples of control and treatment groups. Oxidative stress biomarkers, such as superoxide dismutase, catalase, glutathione peroxidases, ethoxyresorufin O-deethylase, glutathione S-transferase, glutathione reductase, total glutathione and malondialdehyde were investigated in liver and kidney of experimental groups. Histopathological examination of liver, spleen and gut revealed no adverse effects following increasing levels of insect meal inclusions. Hermetia illucens consumption stimulated higher production of neutral than acidic mucins. Altered values of several antioxidant biomarkers indicated an unbalance of oxidative homeostasis in liver and mainly in kidney, linked to the feeding conditions. While lowering of glutathione peroxidase activity may reflect slight adverse effect of HI meals in both tissues, strengthening in levels of ethoxyresorufin O-deethylase, glutathione S-transferase and total glutathione may indicate the shielding of HI meals-mediated oxidative process. Although the administration of both inclusion levels of insect meal did not negatively affect the histological features in fish, the changes of oxidative stress biomarkers advise for an HI dietary inclusion lower than 20%.

Subfields: Aquatic Science, Insect Science, Social Psychology

View Paper: https://www.semanticscholar.org/paper/d546b56d90e56641595f32748c26ea73b72cd5fc

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Aquatic Science

In Insect Science

In Social Psychology

Citations in the corpus

The Utilization of Full-Fat Insect Meal in Rainbow Trout (Oncorhynchus mykiss) Nutrition: The Effects on Growth Performance, Intestinal Microbiota and Gastrointestinal Tract Histomorphology (2019, background): “…components has been observed, including in the nutrition of farm animals, aquaculture and ornamental fish (Belghit et al., 2018; Bruni et al., 2018; Elia et al., 2018; Józefiak D. et al., 2016; Józefiak A. et al., 2018; Kierończyk et al., 2018 a, b; Vargas et al., 2018; Vargas-Abundez et al.,…”
Black soldier fly larvae meal can replace fish meal in diets of sea-water phase Atlantic salmon (Salmo salar) (2019, background): “Replacement of FM with BSF larvae meal in aquafeeds without negative effects on growth or performance has successfully been demonstrated in some fish feeding trials (Belghit et al., 2018a; Cummins et al., 2017; Dumas et al., 2018; Elia et al., 2018; Lock et al., 2016; Magalhães et al., 2017; Renna e”