A review of several reported procedures to determine the degree of N-acetylation for chitin and chitosan using infrared spectroscopy

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Application of Spectroscopic Methods for Structural Analysis of Chitin and Chitosan (2010, methodology): “determination by IR spectroscopy for chitin and chitosan was published by Kasaai [131].”
Functional Characterization of Chitin and Chitosan (2009, neutral): “As is shown in Table 2, various methods have been reported for the determination of chitin and chitosan characteristics [30-45].”
Polysaccharide-Based Hydrogels: The Key Role of Water in Affecting Mechanical Properties (2012, background): “The last region, which is composed of several bands ascribed to the –OH stretching of the polymer glycosidic ring, –OH stretching of water molecules (both free and involved in hydrogen bonds) and different types of amine group stretching, is between 2500 and 3600 cm [37,38].”
Chitosan Nanocomposite Coatings for Food, Paints, and Water Treatment Applications (2019, methodology): “spectroscopy [31–32], elemental analysis [30], Fourier transform infrared (FTIR) spectroscopy [33],”
Characterization and Properties of Chitosan (2011, methodology): “…et al., 1998; Zamani et al., 2008); • HPLC - Ultraviolet (Aiba, 1986; Muzzarelli & Rocchetti, 1985); • Infrared (Baxter et al., 1992; Duarte et al., 2002; Kasaai, 2008; Miya et al., 1980; Moore & Roberts, 1980; Sannan et al., 1978); • 1H nuclear magnetic resonance (Brugnerotto et al., 2001; de…”