Molecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and Transport

By T. Umezawa, K. Nakashima, T. Miyakawa, T. Kuromori, M. Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki. Plant and Cell Physiology, 2010

852 citations recorded by the source corpus.

ABA is a major phytohormone that regulates a broad range of plant traits and is especially important for adaptation to environmental conditions. Our understanding of the molecular basis of ABA responses in plants improved dramatically in 2009 and 2010, banner years for ABA research. There are three major components; PYR/PYL/ RCAR (an ABA receptor), type 2C protein phosphatase (PP2C; a negative regulator) and SNF1-related protein kinase 2 (SnRK2; a positive regulator), and they offer a double negative regulatory system, [PYR/PYL/RCAR—| PP2C—| SnRK2]. In the absence of ABA, PP2C inactivates SnRK2 by direct dephosphorylation. In response to environmental or developmental cues, ABA promotes the interaction of PYR/PYL/RCAR and PP2C, resulting in PP2C inhibition and SnRK2 activation. This signaling complex can work in both the nucleus and cytosol, as it has been shown that SnRK2 phosphorylates basic-domain leucine zipper (bZIP) transcription factors or membrane proteins. Several structural analyses of PYR/PYL/RCAR have provided the mechanistic basis for this ‘core signaling’ model, by elucidating the mechanism of ABA binding of receptors, or the ‘gate–latch–lock’ mechanism of interaction with PP2C in inhibiting activity. On the other hand, intercellular ABA transport had remained a major issue, as had intracellular ABA signaling. Recently, two plasma membrane-type ABC transporters were identified and shed light on the influx/efflux system of ABA, resolving how ABA is transported from cell to cell in plants. Our knowledge of ABA responses in plants has been greatly expanded from intracellular signaling to intercellular transport of ABA.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/ce58d44f3e2dd3472bd6cc59da82faf95e2289c4

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Plant Science

Citations in the corpus

Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks (2012, neutral): “…regulating diverse processes including development and responses to environmental stress and have been shown to regulate stress-induced MAPK and SnRK2 protein kinases negatively (Schweighofer et al., 2004; Cutler et al., 2010; Hubbard et al., 2010; Raghavendra et al., 2010; Umezawa et al., 2010).”
Effects of abiotic stress on plants: a systems biology perspective (2011, neutral): “6, which lacks ABA responses [43].”
NAC transcription factors in plant abiotic stress responses. (2012, background): “Recently, an ABA perception and core signalingmodule, including the ABA receptor (PYR/PYL/RCAR), group A 2C-type protein phosphatase (PP2C), and class III SnRK2 protein kinases was identified [27].”
ABA signaling in stress-response and seed development (2013, background): “PYR/PYL/RCARs and group A PP2Cs are conserved from bryophytes (Umezawa et al. 2010).”
The role of ABA and MAPK signaling pathways in plant abiotic stress responses. (2014, background): “6 triple mutant, and the expression of examined ABA-induced genes was completely blocked (Fujii and Zhu, 2009; Umezawa et al., 2010).”
Advances in Plant Tolerance to Abiotic Stresses (2016, background): “The various physiological reactions regulated by ABA, including stomatal closure, accumulation of osmoprotectants, changes in gene expression, and other phytohormones have been characterized at the molecular level [16].”
Hormone crosstalk in plant disease and defense: more than just jasmonate-salicylate antagonism. (2011, background): “This alleviates negative regulation on PP2C target SnRK2s, leading to activation of ABA signaling (41, 150, 206, 211).”
Smart Engineering of Genetic Resources for Enhanced Salinity Tolerance in Crop Plants (2016, background): “Specific transcription factors mainly regulate the expression of stress-inducible genes; of which members of the characterized families AREB: ABA-responsive elements (ABREs)-binding protein, ABF: ABRE-binding factor, AP2: APETELA2, bZIP: Basic leucine zipper, CBF: core binding factor, NAC: NAM, ATAF”
The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold, and heat (2014, background): “It was revealed that SnRK2, group A PP2Cs, and RCAR/PYR/PYL ABA receptors control the ABA signaling pathway including AREB/ABFs in land plants (reviewed in Umezawa et al., 2010; Miyakawa et al., 2013; Nakashima and Yamaguchi-Shinozaki, 2013).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “In addition to their role in growth and development, the impact of phytohormones on abiotic stress tolerance has been well established (Peleg and Blumwald, 2010; Umezawa et al., 2010; Hadiarto and Tran, 2011; Nishiyama et al., 2011).”