Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink

By Falguni Pati, Jinah Jang, Dong-Heon Ha, Sung Won Kim, J. Rhie, J. Shim, Deok‐Ho Kim, Dong-Woo Cho. Nature Communications, 2014

1,709 citations recorded by the source corpus.

The ability to print and pattern all the components that make up a tissue (cells and matrix materials) in three dimensions to generate structures similar to tissues is an exciting prospect of bioprinting. However, the majority of the matrix materials used so far for bioprinting cannot represent the complexity of natural extracellular matrix (ECM) and thus are unable to reconstitute the intrinsic cellular morphologies and functions. Here, we develop a method for the bioprinting of cell-laden constructs with novel decellularized extracellular matrix (dECM) bioink capable of providing an optimized microenvironment conducive to the growth of three-dimensional structured tissue. We show the versatility and flexibility of the developed bioprinting process using tissue-specific dECM bioinks, including adipose, cartilage and heart tissues, capable of providing crucial cues for cells engraftment, survival and long-term function. We achieve high cell viability and functionality of the printed dECM structures using our bioprinting method. The application of 3D printing to biomaterials presents interesting possibilities for tissue engineering. Here, the authors show that a printing medium derived from an extracellular matrix can be applied to printing tissue analogues with enhanced cell compatibility.

Subfields: Biomedical Engineering, Molecular Biology, Surgery

View Paper: https://www.semanticscholar.org/paper/ce42256e8949d4c522eebeda749d70903999865a

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Citations in the corpus

Reinforcement of hydrogels using three-dimensionally printed microfibres (2015, neutral): “Recent composite systems include non-woven scaffolds manufactured via solution-electrospinning techniques 9,24–27 and scaffolds fabricated via 3D-printing 28–33 .”
Biofabrication: A Guide to Technology and Terminology. (2017, background): “In addition, hydrogels that encapsulate biological components could be deposited together with thermoplastic materials, thereby circumventing the limitations [33,34] imposed by the high temperatures.”
3D Bioprinting for Engineering Complex Tissues (2015, background): “Recently, Pati et al. (2014) showed that dECMs from three tissues could be solubilized into bioinks and bioprinted.”
Recent trends in bioinks for 3D printing (2018, background): “The investigation showed high cell viability and functionality of the constructs after the printing [100].”
Current Status of Bioinks for Micro-Extrusion-Based 3D Bioprinting (2016, background): “Decellularized ECM Provide native microenvironment to laden cells Low viscosity and poor resolution [59,60]”