Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types.

By J. Kelm, Nicholas E. Timmins, Catherine J. Brown, M. Fussenegger, L. Nielsen. Biotechnology and Bioengineering, 2003

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Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME (2013, positive): “canaliculi and dense cell–cell contacts (Kelm et al. 2003).”
Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture (2018, methodology): “Although non-microfluidic methods [25, 28, 38, 41-55] for spheroid formation and”
Protease-dependent versus -independent cancer cell invasion programs: three-dimensional amoeboid movement revisited (2009, methodology): “(A) Di-I–labeled multicellular spheroids (150– 200 μm in diameter) of HT-1080 cells or MDAMB-231 cells were prepared in hanging droplets (Kelm et al., 2003) after electroporation with a control siRNA, MMP-1 and MMP-2 siRNAs, MT1 siRNA (50–100 nM; siRNA sequences can be found in Li et al.”
Spherical Cancer Models in Tumor Biology (2015, methodology): “The InSphero AG (Schlieren, Switzerland) plate system permits simple transfer of the MCTS from the drop in the well for drug incubation [9,55].”
Opportunities and Challenges for use of Tumor Spheroids as Models to Test Drug Delivery and Efficacy (2012, neutral): “hanging drops, culture of cells on non-adherent surfaces, spinner flask cultures, and NASA rotary cell culture systems [9,15,43–48].”