Direct Observation of Protein Solvation and Discrete Disorder with Experimental Crystallographic Phases

By F. T. Burling, William I. Weis, K. Flaherty, A. Brünger. 1996

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Citations in the corpus

Using SAD data in Phaser (2011, neutral): “(b) A model of the four Yb atoms in the Yb-substituted mannosebinding protein (Burling et al., 1996) was refined in Phaser with isotropic B factors before computing an LLG map, which illustrates the positive and negative features that indicate anisotropic motion.”
Application of DEN refinement and automated model building to a difficult case of molecular-replacement phasing: the structure of a putative succinyl-diaminopimelate desuccinylase from Corynebacterium glutamicum (2012, methodology): “…point and f 0 = 3.32 ( 3.59), f 00 = 3.66 (1.05) at the remote wavelength, where the numbers refer to the results from the Friedel mate F to Freference lack-of-closure expressions and the numbers in parentheses refer to the F to Freference lack-of-closure expressions (Burling et al., 1996).”
Modelling dynamics in protein crystal structures by ensemble refinement (2012, unknown): “These deviations decreased for the ensemble models by 0.002 Å, 0.26° and 6.6° respectively, when considering the centroids of the observed stere-ochemical distributions.”
Experimental assessment of differences between related protein crystal structures. (1999, methodology): “D55, 1878±1884 Kleywegt Experimental assessment of structural differences 1881 Figure 1 (a) Local density-correlation map for molecule A of mannose-binding protein, calculated from the MAD-phased experimental electron-density map (Burling et al., 1996).”
Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain (2004, methodology): “The current tubulin–colchicine:RB3-SLD complex structure has been determined at higher resolution using experimental phases to avoid model bia”
Interpretation of ensembles created by multiple iterative rebuilding of macromolecular models (2007, background): “…of many slightly different conformations and arrangements of the macromolecules within the crystal itself (Kuriyan et al., 1986; Gros et al., 1990; Burling et al., 1996; Burling & Brünger, 1994; Clarage & Phillips, 1994; Pellegrini et al., 1997; Chen & Chapman, 2001; Vitkup et al., 2002;…”