Campylobacter jejuni Binds Intestinal H(O) Antigen (Fucα1, 2Galβ1, 4GlcNAc), and Fucosyloligosaccharides of Human Milk Inhibit Its Binding and Infection*
By G. Ruiz-Palacios, L. Cervantes, P. Ramos, B. Chávez-Munguía, D. Newburg. Journal of Biological Chemistry, 2003
605 citations recorded by the source corpus.
The most common cause of infant mortality is diarrhea; the most common cause of bacterial diarrhea isCampylobacter jejuni, which is also the primary cause of motor neuron paralysis. The first step in campylobacter pathogenesis is adherence to intestinal mucosa. We found that such binding was inhibited in vitro by human milk and, with high avidity, by α1,2-fucosylated carbohydrate moieties containing the H(O) blood group epitope (Fucα1,2Galβ1,4GlcNAc … ). In studies on the mechanism of adherence, campylobacter, which normally does not bind to Chinese hamster ovary cells, bound avidly when the cells were transfected with a human α1,2-fucosyltransferase gene that caused overexpression of H-2 antigen; binding was specifically inhibited by H-2 ligands (lectins Ulex europaeus and Lotus tetragonolobus and H-2 monoclonal antibody), H-2 mimetics, and human milk oligosaccharides. Human milk oligosaccharides inhibited campylobacter colonization of mice in vivo and human intestinal mucosa ex vivo. Campylobacter colonization of nursing mouse pups was inhibited if their dams had been transfected with a human α1,2-fucosyltransferase gene that caused expression of H(O) antigen in milk. We conclude that campylobacter binding to intestinal H-2 antigen is essential for infection. Milk fucosyloligosaccharides and specific fucosyl α1,2-linked molecules inhibit this binding and may represent a novel class of antimicrobial agents.
Subfields: Endocrinology, Infectious Diseases, Nutrition and Dietetics
View Paper: https://www.semanticscholar.org/paper/cbf44b2324d1e018d13b49defbbd9df48a09e45d
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In Nutrition and Dietetics
Citations in the corpus
- Human milk glycobiome and its impact on the infant gastrointestinal microbiota (2010, background): “These specific HMOs are likely candidates for the important biological roles of milk in maintaining a healthy gut microbiota and in the prevention of pathogenic diseases among infants, as previously reported (6, 11, 18, 35).”
- Mechanisms and consequences of intestinal dysbiosis (2017, background): “Changes in the density of fucosylated glycans or the passage of soluble fucosylated molecules, such as milk oligosaccharides [113] in the intestinal lumen alter the adhesion of C.”
- Human milk oligosaccharides: every baby needs a sugar mama. (2012, background): “The most comprehensive data on HMO as antiadhesive antimicrobials has been reported for Campylobacter jejuni infections, which are one of the most common causes of bacterial diarrhea and infant mortality (Ruiz-Palacios et al. 2003; Morrow et al. 2004).”