Enzyme immobilization on electrospun polymer nanofibers: An overview

By Zhen‐Gang Wang, L. Wan, Zhenmei Liu, Xiaojun Huang, Zhi‐Kang Xu. 2009

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Enzyme immobilization: an overview on techniques and support materials (2012, background): “-collagen fibers proved to be excellent supporting matrix for catalase immobilization by retaining significant activity even after 26 reuses (Chen et al. 2011b).”
Chitosan–halloysite hybrid-nanotubes: Horseradish peroxidase immobilization and applications in phenol removal (2013, background): “To make enzymes cost-effective, long-lived, and highly active, various supports have been utilized to immobilize enzymes [1–3].”
Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning (2011, background): “Enzyme immobilization: Electrospun nanofibers have been proven to be excellent supports for enzyme immobilization because they can provide large surface area-to-volume ratios, pore sizes tailored to protein molecule dimensions, functionalized surfaces, multiple sites for interaction or attachment, a”
Recent advance in the support and technology used in enzyme immobilization (2009, methodology): “As for covalent immobilization under microwave irradiation, macromolecules crowding was combined with small molecular quenching to perfect microwave-assisted covalent immobilization (Wang et al., 2009a).”
A general overview of support materials for enzyme immobilization: Characteristics, properties, practical utility (2018, background): “They also have high porosity and surface areas, which lead to high enzyme loading [130].”