Enzyme immobilization: an overview on techniques and support materials

By Sumitra Datta, bullet L Rene, Christena bullet, Yamuna Rani, S. Rajaram. 3 Biotech, 2012

1,259 citations recorded by the source corpus.

The current demands of the world’s biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. These requirements are inevitable to facilitate large-scale and economic formulation. Enzyme immobilization provides an excellent base for increasing availability of enzyme to the substrate with greater turnover over a considerable period of time. Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization. Nowadays, immobilized enzymes are preferred over their free counterpart due to their prolonged availability that curtails redundant downstream and purification processes. Future investigations should endeavor at adopting logistic and sensible entrapment techniques along with innovatively modified supports to improve the state of enzyme immobilization and provide new perspectives to the industrial sector.

Subfields: Biomedical Engineering, Biotechnology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/783d648e690d3adff79689350557ffb221d6163d

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In Biomedical Engineering

1. R. Singh
2. J. Knowles
3. M. AlQaysi
4. F. Foroutan
5. Chinmaya Mahapatra
6. Gareth J. Owens
7. H. Kim
8. Cheol-Min Han

Citations in the corpus

• Enhanced biocatalytic sustainability of laccase by immobilization on functionalized carbon nanotubes/polysulfone membranes (2019, background): “One of the smartest uses of immobilized enzymes is their application in the treatment of wastes, including waste waters [2–6] where both the specific activity and possibility of recycle are combined for maximum performance [7].”
• Sol-gel based materials for biomedical applications (2016, background): “Other approaches include the ability to make the matrices either electrochemically active or optically transparent, the ability to achieve high protein loading, to tune the polarity and porosity of the matrix to selectively modulate enzyme activity, the potential to modulate the material composition”
• Microfluidics Integrated Biosensors: A Leading Technology towards Lab-on-a-Chip and Sensing Applications (2015, background): “In essence, the main advantages of an immobilized biological recognition element include easy separation from the reaction mixture and the ability to control reaction times and minimize the biological recognition elements lost in the reaction mixture [16].”
• Extremophiles: An Overview of Microorganism from Extreme Environment (2014, background): “, 2001), chemical modification (Tyagi and Gupta, 1998; Gaur and Khare, 2009; Gupta and Khare, 2009), directed evolution (Arnold and Moore, 1998) and immobilization (Gaur et al., 2008; Datta et al., 2013).”
• An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes (2015, neutral): “[36] In some cases, affinity binding is also included as one of the physical methods for immobilization of enzymes.”