A one-step homogeneous immunoassay for cancer biomarker detection using gold nanoparticle probes coupled with dynamic light scattering.

By Xiong Liu, Q. Dai, Lauren A Austin, Janelle L. Coutts, G. Knowles, J. Zou, Hui Chen, Q. Huo. Journal of the American Chemical Society, 2008

636 citations recorded by the source corpus.

A one-step homogeneous immunoassay for the detection of a prostate cancer biomarker, free-PSA (prostate specific antigen), was developed using gold nanoparticle probes coupled with dynamic light scattering (DLS) measurements. A spherical gold nanoparticle with a core diameter around 37 nm and a gold nanorod with a dimension of 40 by 10 nm were first conjugated with two different primary anti-PSA antibodies and then used as optical probes for the immunoassay. In the presence of antigen f-PSA in solution, the nanoparticles and nanorods aggregate together into pairs and oligomers through the formation of a sandwich type antibody-antigen-antibody linkage. The relative ratio of nanoparticle-nanorod pairs and oligomers versus individual nanoparticles was quantitatively monitored by DLS measurement. A correlation can be established between this relative ratio and the amount of antigen in solution. The light scattering intensity of nanoparticles and nanoparticle oligomers is several orders of magnitude higher than proteins and other typical molecules, making it possible to detect nanoparticle probes in the low picomolar concentration range. f-PSA in the concentration range from 0.1 to 10 ng/mL was detected by this one-step and washing-free homogeneous immunoassay.

Subfields: Biomedical Engineering, Electronic, Optical and Magnetic Materials, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ca4289c31334189d64446b81b851f656125ad909

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In Molecular Biology

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Citations in the corpus

• Nanotechnology for Early Cancer Detection (2010, background): “This enables biomarker detection at very low concentrations [48].”
• Applications of gold nanoparticles in cancer nanotechnology (2008, neutral): “Gold nanoparticles have also been employed for many other applications such as immunoassay (Hirsch et al 2003a, 2005; Liu et al 2008), protein assay (Tang et al 2007), time-of-fl ight secondary ion mass spectrometry (Kim et al 2006), capillary electrophoresis (Tseng et al 2005), and detection of…”
• Dynamic light scattering for gold nanorod size characterization and study of nanorod–protein interactions (2012, methodology): “Based on the nanoparticle size change, we and others have developed a novel platform technology, nanoparticle-enabled dynamic light scattering assay (NanoDLSayTM) for biological and chemical detection and analysis with high to ultrahigh sensitivity and excellent reproducibility [32–38].”
• A Review of Current Research into the Biogenic Synthesis of Metal and Metal Oxide Nanoparticles via Marine Algae and Seagrasses (2017, background): “The magnetic properties of the magnetite nanoparticles make them ideal candidates for magnetic resonance imaging (MRI) and targeted drug delivery applications [16, 151].”