Activating transcription factor 3 (ATF3) induction by axotomy in sensory and motoneurons: A novel neuronal marker of nerve injury.

By H. Tsujino, E. Kondo, T. Fukuoka, Yi Dai, A. Tokunaga, K. Miki, K. Yonenobu, Takahiro Ochi, K. Noguchi. Molecular and cellular neurosciences, 2000

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p38 Mitogen-Activated Protein Kinase Is Activated after a Spinal Nerve Ligation in Spinal Cord Microglia and Dorsal Root Ganglion Neurons and Contributes to the Generation of Neuropathic Pain (2003, background): “p38 activation in DRG neurons may contribute to the increased expression of many proteins, such as BDNF, 2 calcium channel, and activating transcription factor 3, that occur in injured DRG neurons (Zhou et al., 1999; Tsujino et al., 2000; Luo et al., 2001).”
Microarray analysis after RNA amplification can detect pronounced differences in gene expression using limma (2006, methodology): “In brief, the Affymetrix standard protocol [ 8 ] (in the article referred to as the OneRA protocol) was used to generate three labelled cRNA samples from each tissue pool using 5 μg of total RNA as starting material (Figure 1a).”