Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection.

By Y. Cao, R. Jin, Chad A. Mirkin. Science, 2002

2,772 citations recorded by the source corpus.

Multiplexed detection of oligonucleotide targets has been performed with gold nanoparticle probes labeled with oligonucleotides and Raman-active dyes. The gold nanoparticles facilitate the formation of a silver coating that acts as a surface-enhanced Raman scattering promoter for the dye-labeled particles that have been captured by target molecules and an underlying chip in microarray format. The strategy provides the high-sensitivity and high-selectivity attributes of gray-scale scanometric detection but adds multiplexing and ratioing capabilities because a very large number of probes can be designed based on the concept of using a Raman tag as a narrow-band spectroscopic fingerprint. Six dissimilar DNA targets with six Raman-labeled nanoparticle probes were distinguished, as well as two RNA targets with single nucleotide polymorphisms. The current unoptimized detection limit of this method is 20 femtomolar.

Subfields: Biomedical Engineering, Electronic, Optical and Magnetic Materials, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/c828a5c7963d021dd9c9b8e8408be741575c29df

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In Biomedical Engineering

In Electronic, Optical and Magnetic Materials

In Molecular Biology

Citations in the corpus

Applications of gold nanoparticles in cancer nanotechnology (2008, neutral): “…force microscopy (AFM) (Han et al 2000; Jin et al 2007), gel electrophoresis (Qin and Yung 2007), scanometric assay (Son and Lee 2007), chro-nocoulometry (Zhang et al 2007), ampliﬁ ed voltammetric detection (Wang et al 2008a), SPR imaging (Li et al 2006), and Raman spectroscopy (Cao et al 2002).”