Recombinant Pfs230, a Plasmodium falciparum gametocyte protein, induces antisera that reduce the infectivity of Plasmodium falciparum to mosquitoes.

By K. Williamson, David B. Keister, Olga V Muratova, D. Kaslow. 1995

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Citations in the corpus

• Malaria transmission‐blocking antigen, Pfs230, mediates human red blood cell binding to exflagellating male parasites and oocyst production (2006, methodology): “The parasite pellet was extracted in NETT [150 mM NaCl/5 mM EDTA/0.5% Triton-X 100/50 mM Tris (pH 8.0)] and analysed directly by SDS-PAGE or assayed by immunoprecipitation with the indicated antibody as described previously (Williamson et al., 1995).”
• Structural and Immunological Characterization of Recombinant 6-Cysteine Domains of the Plasmodium falciparum Sexual Stage Protein Pfs230* (2016, background): “(23) and more recently by Tachibana et al.”
• N-Terminal Prodomain of Pfs230 Synthesized Using a Cell-Free System Is Sufficient To Induce Complement-Dependent Malaria Transmission-Blocking Activity (2011, background): “falciparum to mosquitoes in the presence of complement (43).”
• Identification of domains within Pfs230 that elicit transmission blocking antibody responses (2019, background): “In addition to the above constructs which covered the entire region of the cleaved Pfs230 (aa 443–3135) [8], we also evaluated mouse IgG against prodomain (aa 22–588) of the Pfs230 recombinant protein fragment.”
• Worldwide sequence conservation of transmission-blocking vaccine candidate Pvs230 in Plasmodium vivax (2011, unknown): “falciparum parasites to infect mosquitoes [26].”
• Three Members of the 6-cys Protein Family of Plasmodium Play a Role in Gamete Fertility (2010, background): “Indeed specific antibodies against the sexual stages of the human parasite Plasmodium falciparum, P48/45 and P230 can prevent zygote formation and thus block transmission of the parasite [19,20,21,22,23,24,25,26].”
• Plasmodium 6-Cysteine Proteins: Functional Diversity, Transmission-Blocking Antibodies and Structural Scaffolds (2022, methodology): “MBP was used for the early expression of Pfs230 fragments (Williamson et al., 1993; Riley et al., 1995; Williamson et al., 1995; Milek et al., 1998; Bustamante et al., 2000) and GST for Pfs48/45 fragments (Kocken et al.”
• The s48/45 six-cysteine proteins: mediators of interaction throughout the Plasmodium life cycle (2016, background): “…and elicit full transmission-blocking immunity, making P230 a challenging protein to produce in heterologous expression systems (Riley et al., 1995; Williamson et al., 1995; Vincent et al., 1999; Bustamante et al., 2000; Williamson, 2003; Farrance et al., 2011; Tachibana et al., 2011; Kapulu et…”