SIZ1-Mediated Sumoylation of ICE1 Controls CBF3/DREB1A Expression and Freezing Tolerance in Arabidopsis[W][OA]

By K. Miura, Jing Bo Jin, Jiyoung Lee, Chan Yul Yoo, V. Stirm, Tomoko Miura, E. Ashworth, R. Bressan, D. Yun, P. Hasegawa. The Plant Cell Online, 2007

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SIZ1 is a SUMO E3 ligase that facilitates conjugation of SUMO to protein substrates. siz1-2 and siz1-3 T-DNA insertion alleles that caused freezing and chilling sensitivities were complemented genetically by expressing SIZ1, indicating that the SIZ1 is a controller of low temperature adaptation in plants. Cold-induced expression of CBF/DREB1, particularly of CBF3/DREB1A, and of the regulon genes was repressed by siz1. siz1 did not affect expression of ICE1, which encodes a MYC transcription factor that is a controller of CBF3/DREB1A. A K393R substitution in ICE1 [ICE1(K393R)] blocked SIZ1-mediated sumoylation in vitro and in protoplasts identifying the K393 residue as the principal site of SUMO conjugation. SIZ1-dependent sumoylation of ICE1 in protoplasts was moderately induced by cold. Sumoylation of recombinant ICE1 reduced polyubiquitination of the protein in vitro. ICE1(K393R) expression in wild-type plants repressed cold-induced CBF3/DREB1A expression and increased freezing sensitivity. Furthermore, expression of ICE1(K393R) induced transcript accumulation of MYB15, which encodes a MYB transcription factor that is a negative regulator of CBF/DREB1. SIZ1-dependent sumoylation of ICE1 may activate and/or stabilize the protein, facilitating expression of CBF3/DREB1A and repression of MYB15, leading to low temperature tolerance.

Subfields: Biochemistry, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/c5bce9e8610529fc6a086fbc5613841d7c1fbb91

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Citations in the corpus

Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L. (2013, background): “For instance, the expression levels of ICE1 and CBF3 can be enhanced in different freezing temperatures [40].”
Cold stress regulation of gene expression in plants. (2007, background): “Recently, a role for sumoylation in cold acclimation was found through studies on the Arabidopsis SUMO E3 ligase, SIZ1 (SAP and Miz1) [ 70 ].”
Role of DREBs in regulation of abiotic stress responses in plants. (2011, background): “ICE1 (Miura et al., 2007), where sumoylation is a process that conjugates SUMO (small ubiquitin-related modifier) to a protein substrate.”
Molecular Basis of Plant Cold Acclimation: Insights Gained from Studying the CBF Cold Response Pathway1 (2010, background): “The results of Miura et al. (2007) provide strong evidence that this is the case and that this is accomplished through low temperature-induced sumoylation of ICE1 mediated by the SIZ1 protein, a SUMO E3 ligase.”
Over-expression of miR397 improves plant tolerance to cold stress in Arabidopsis thaliana (2014, background): “2006) and sumoylation (Miura et al. 2007).”
Jasmonate Regulates the INDUCER OF CBF EXPRESSION–C-REPEAT BINDING FACTOR/DRE BINDING FACTOR1 Cascade and Freezing Tolerance in Arabidopsis[W] (2013, background): “Moreover, the SMALL UBIQUITINRELATED MODIFIER E3 ligase SIZ1 (SAP and Miz) and the ubiquitin E3 ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 have been shown to modify ICE1 posttranslationally and function in the ICE-CBF/DREB1 signaling pathway (Dong et al., 2006; Miura et al., 2007).”
Plant abiotic stress response and nutrient use efficiency (2020, background): “ICE1 is also sumoylated (and thereby stabilized) by a SUMO E3 ligase, SIZ1 (SAP/Miz) (Miura et al., 2007) in plant response to low temperature.”
Genomics of Cold Hardiness in Woody Plants (2014, background): “A role for Vitis CBFs in plant developmental processes has not been investigated extensively.”
Signal transduction during cold, salt, and drought stresses in plants (2011, neutral): “its stability at cold [47].”
Ethylene Signaling Negatively Regulates Freezing Tolerance by Repressing Expression of CBF and Type-A ARR Genes in Arabidopsis[W][OA] (2012, background): “Cold stress activates CBF expression (Gilmour et al., 1998; Liu et al., 1998) and induces the expression of a large subset of COR genes (Maruyama et al., 2004; Vogel et al., 2005), and the overexpression of CBFs leads to constitutively enhanced freezing tolerance (Jaglo-Ottosen et al., 1998; Liu et ”
miR394 and its target gene LCR are involved in cold stress response in Arabidopsis (2016, methodology): “The survival experiment was performed based on the method of Miura et al. (2007).”
Abiotic stress response in plants : when post-transcriptional and post-translational regulations control transcription (2008, background): “A general accumulation of SUMO conjugates is an early effect of the exposure to extreme temperatures, oxidative cues and dehydration stress [29,106,108,109,111,112].”